Optimizing tick artificial membrane feeding for Ixodes scapularis.
Optimizing tick artificial membrane feeding for Ixodes scapularis.
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优化为ixodes capularis的壁虱人造膜喂养。
DOI：
10.1038/s41598-023-43200-z
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发表时间：
2023-09-27
影响因子：
4.6
通讯作者：
Ribeiro, Jose M.
中科院分区：
文献类型：
--
作者：
Guizzo, Melina Garcia;Meneses, Claudio;Cecilio, Pedro Amado;Alvarenga, Patricia Hessab;Sonenshine, Daniel;Ribeiro, Jose M.
人工膜喂养（AMF）是一种强大且多功能的技术，在疾病媒介物种的研究中具有广泛的应用。自首次描述以来，AMF 一直在针对不同蜱种和生命阶段进行不断优化和标准化。在美国，肩胛硬蜱是蜱传人畜共患病的主要媒介，包括引起人类和动物莱姆病的病原体。为了提高人工喂养的肩胛 I. scapularis 成年雌性的整体健康状况，我们优化了 AMF 技术，与之前的研究中描述的相比，显着提高了附着率、肿胀成功率、产卵和孵化率。诸如膜厚度和蜱虫所接触的光/暗周期等参数都经过细化，以更准确地反映蜱虫的自然行为和生命周期。此外，蜱只以血液、血液++ATP或血液++ATP+庆大霉素为食。仅以血液人工喂养蜱是成功的，并产生了能够自然喂养宿主（即小鼠）的后代。添加 ATP 作为摄食刺激剂并不能改善蜱的附着或肿胀。值得注意的是，使用庆大霉素（一种常用于蜱 AMF 中以防止微生物污染的抗生素）会对人工喂养蜱后代中的布氏立克次体内共生体水平产生负面影响。此外，与人工仅以血液喂养的蜱相比，以庆大霉素喂养的蜱的产卵成功率有所下降，这阻碍了 AMF 中抗生素的使用。总体而言，我们的数据表明，仅对成年雌性蜱虫进行血液 AMF 结合其后代对小鼠的自然喂养，可能会被用作蜱虫饲养的综合方法，从而消除《动物福利法》(AWA) 下受保护物种的使用。值得注意的是，虽然针对肩胛蜱成虫进行了优化，但肩胛蜱若虫、其他蜱种和白蛉也可以使用本研究中描述的膜进行喂养，这表明它可能是人工喂养多种食血物种的合适替代品。
Artificial membrane feeding (AMF) is a powerful and versatile technique with a wide range of applications in the study of disease vectors species. Since its first description, AMF has been under constant optimization and standardization for different tick species and life stages. In the USA, Ixodes scapularis is the main vector of tick-borne zoonoses including the pathogens causing Lyme disease in humans and animals. Seeking to improve the overall fitness of I. scapularis adult females fed artificially, here, we have optimized the AMF technique, considerably enhancing attachment rate, engorgement success, egg laying, and egg hatching compared to those described in previous studies. Parameters such as the membrane thickness and the light/dark cycle to which the ticks were exposed were refined to more closely reflect the tick’s natural behavior and life cycle. Additionally, ticks were fed on blood only, blood + ATP or blood + ATP + gentamicin. The artificial feeding of ticks on blood only was successful and generated a progeny capable of feeding naturally on a host, i.e., mice. Adding ATP as a feeding stimulant did not improve tick attachment or engorgement. Notably, the administration of gentamicin, an antibiotic commonly used in tick AMF to prevent microbial contamination, negatively impacted Rickettsia buchneri endosymbiont levels in the progeny of artificially fed ticks. In addition, gentamicin-fed ticks showed a reduction in oviposition success compared to ticks artificially fed on blood only, discouraging the use of antibiotics in AMF. Overall, our data suggest that the AMF of adult females on blood only, in association with the natural feeding of their progeny on mice, might be used as an integrated approach in tick rearing, eliminating the use of protected species under the Animal Welfare Act (AWA). Of note, although optimized for I. scapularis adult ticks, I. scapularis nymphs, other tick species, and sand flies could also be fed using the membrane described in this study, indicating that it might be a suitable alternative for the artificial feeding of a variety of hematophagous species.
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影响因子：
16.6
作者：
Gulia-Nuss M;Nuss AB;Meyer JM;Sonenshine DE;Roe RM;Waterhouse RM;Sattelle DB;de la Fuente J;Ribeiro JM;Megy K;Thimmapuram J;Miller JR;Walenz BP;Koren S;Hostetler JB;Thiagarajan M;Joardar VS;Hannick LI;Bidwell S;Hammond MP;Young S;Zeng Q;Abrudan JL;Almeida FC;Ayllón N;Bhide K;Bissinger BW;Bonzon-Kulichenko E;Buckingham SD;Caffrey DR;Caimano MJ;Croset V;Driscoll T;Gilbert D;Gillespie JJ;Giraldo-Calderón GI;Grabowski JM;Jiang D;Khalil SMS;Kim D;Kocan KM;Koči J;Kuhn RJ;Kurtti TJ;Lees K;Lang EG;Kennedy RC;Kwon H;Perera R;Qi Y;Radolf JD;Sakamoto JM;Sánchez-Gracia A;Severo MS;Silverman N;Šimo L;Tojo M;Tornador C;Van Zee JP;Vázquez J;Vieira FG;Villar M;Wespiser AR;Yang Y;Zhu J;Arensburger P;Pietrantonio PV;Barker SC;Shao R;Zdobnov EM;Hauser F;Grimmelikhuijzen CJP;Park Y;Rozas J;Benton R;Pedra JHF;Nelson DR;Unger MF;Tubio JMC;Tu Z;Robertson HM;Shumway M;Sutton G;Wortman JR;Lawson D;Wikel SK;Nene VM;Fraser CM;Collins FH;Birren B;Nelson KE;Caler E;Hill CA
通讯作者：
Hill CA
影响因子：
3.8
作者：
通讯作者：
--
影响因子：
3.2
作者：
Al-Khafaji, Alaa M.;Armstrong, Stuart D.;Makepeace, Benjamin L.
通讯作者：
Makepeace, Benjamin L.
影响因子：
2.1
作者：
DAVIS, EL;BUTLER, JF;KLINE, DL
通讯作者：
KLINE, DL
影响因子：
3.2
作者：
Krull, Christoph;Boehme, Bettina;Nijhof, Ard M.
通讯作者：
Nijhof, Ard M.