Cellular transformation by coordinated action of three peptide growth factors from human platelets
Cellular transformation by coordinated action of three peptide growth factors from human platelets
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人血小板中三种肽生长因子协调作用的细胞转化
作者：
R. Assoian;Gary R. Grotendorst;Dorothea M. Miller;M. Sporn
血小板衍生生长因子1-4(PDGF)参与调节结缔组织细胞的有丝分裂，最近的研究也表明它可能在介导细胞转化中发挥作用。猴肉瘤病毒sis携带的癌基因与PDGF5-8的一链同源，用这种生长因子处理非肿瘤细胞会导致另一癌基因myc(ref.9)。PDGF还刺激转化细胞所特有的蛋白质的合成。然而，表型转化似乎不是PDGF单独作用的结果。例如，在缺乏其他癌基因表达的情况下，myc的表达不会转化细胞11。我们最近发现，除了血小板衍生生长因子外，血小板还含有另一种多肽生长因子，转化生长因子-β(转化生长因子-β)12，13。我们在此报道，人血小板提取物可以诱导非肿瘤性大鼠肾成纤维细胞(NRK)非贴壁生长，但纯化的PDGF单独不能诱导这种作用。相反，血小板提取物的转化活性是由于三种不同的肽的协同作用：PDGF、转化生长因子-β和一种新发现的表皮生长因子类似物。
Platelet-derived growth factor1–4 (PDGF) is known to be involved in regulating the mitosis of connective tissue cells, and recent studies have also shown that it may function in mediating cellular transformation5–8. The oncogene carried by simian sarcoma virus, sis, is homologous to one chain of PDGF5–8, and treatment of non-neoplastic cells with this growth factor results in increased transcription of another oncogene, myc (ref. 9). PDGF also stimulates the synthesis of proteins that are characteristic of transformed cells10. However, phenotypic transformation does not appear to result from the action of PDGF alone. For example, expression of myc does not transform cells in the absence of other oncogene expression11. We have recently shown that platelets contain another peptide growth factor, transforming growth factor-β (TGF-β)12,13, in addition to PDGF. We report here that extracts of human platelets can induce anchorage-independent growth of non-neoplastic rat kidney (NRK) fibroblasts, but that purified PDGF alone does not elicit this effect. Rather, the transforming activity of the platelet extract is due to a concerted action of three distinct peptides: PDGF, TGF-β and a newly identified analogue of epidermal growth factor (EGF).
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在培养的成纤维细胞中，血小板衍生生长因子对表皮生长因子（EGF）的有丝分裂反应。
影响因子：
3.7
作者：
Wharton,W;Leof,E;Olashaw,N;O'Keefe,EJ;Pledger,WJ
通讯作者：
Pledger,WJ
表皮生长因子的非有丝分裂类似物增强内源性膜蛋白的磷酸化。
DOI：
10.1016/0006-291x(81)91290-0
发表时间：
1981
影响因子：
3.1
作者：
Schreiber,AB;Yarden,Y;Schlessinger,J
通讯作者：
Schlessinger,J
DOI：
10.1073/pnas.79.17.5312
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHILDS, CB;PROPER, JA;MOSES, HL
通讯作者：
MOSES, HL
DOI：
10.1073/pnas.76.4.1809
发表时间：
1979-01-01
影响因子：
11.1
作者：
ANTONIADES, HN;SCHER, CD;STILES, CD
通讯作者：
STILES, CD
影响因子：
56.9
作者：
DOOLITTLE, RF;HUNKAPILLER, MW;ANTONIADES, HN
通讯作者：
ANTONIADES, HN