Pharmacologically relevant intake during chronic, free-choice drinking rhythms in selectively bred high alcohol-preferring mice.
Pharmacologically relevant intake during chronic, free-choice drinking rhythms in selectively bred high alcohol-preferring mice.
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DOI：
10.1111/j.1369-1600.2011.00412.x
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发表时间：
2013-11
影响因子：
3.4
通讯作者：
Grahame NJ
中科院分区：
文献类型：
--
作者：
Matson LM;Grahame NJ
在四周的时间里，研究人员选择性地培育了多株高酒精偏好（HAP）小鼠，让它们每天24小时摄入10%的乙醇（v/v），最高的饮酒量超过20 g/kg/天。我们观察了昼夜节律饮酒模式和由此产生的血液乙醇浓度在HAP系。我们还比较了最高饮用HAP系与C57BL/6J （B6）自交系的饮酒节律和相应的血乙醇浓度（BEC）。成年雄性和雌性杂交HAP (cHAP)， HAP重复系1、2、3和B6小鼠在光-暗循环的黑暗部分每两小时评估一次摄入量之前，可以自由选择获得10%乙醇和水3周。所有的HAP系都达到并保持了高于HAP1小鼠代谢酒精的速度的酒精摄入量，BECs与这一发现一致。此外，cHAP和HAP1小鼠在整个周期的黑暗部分保持过量的摄入水平，累积的平均BEC水平分别为261.5±18.09和217.9±25.02 mg/dl。B6小鼠饮酒相对适度，BEC水平不高（53.63 + 8.15 mg/dl）。HAP1和cHAP基因系显示的自由选择饮酒可能为模拟酒精依赖个体经常发生的过量饮酒提供了独特的机会，并允许探索过度饮酒的易感性因素，以及酒精暴露后生理、行为和毒理学结果的发展。
Multiple lines of High Alcohol Preferring (HAP) mice were selectively bred for their intake of 10% ethanol (v/v) during 24-h daily access over a four-week period, with the highest drinking lines exhibiting intakes in excess of 20 g/kg/day. We observed circadian drinking patterns and resulting blood ethanol concentrations in the HAP lines. We also compared the drinking rhythms and corresponding blood ethanol concentrations (BEC) of the highest drinking HAP lines to those of the C57BL/6J (B6) inbred strain. Adult male and female crossed HAP (cHAP), HAP replicate lines 1, 2, 3, and B6 mice had free-choice access to 10% ethanol and water for 3 weeks prior to bi-hourly assessments of intake throughout the dark portion of the light-dark cycle. All HAP lines reached and maintained a rate of alcohol intake above the rate at which HAP1 mice metabolize alcohol, and BECs were consistent with this finding. Further, cHAP and HAP1 mice maintained an excessive level of intake throughout the dark portion of the cycle, accumulating mean BEC levels of 261.5 ± 18.09 and 217.9 ± 25.02 mg/dl, respectively. B6 mice drank comparatively modestly, and did not accumulate high BEC levels (53.63 + 8.15 mg/dl). Free-choice drinking demonstrated by the HAP1 and cHAP lines may provide a unique opportunity for modeling the excessive intake that often occurs in alcohol-dependent individuals, and allow for exploration of predisposing factors for excessive consumption, as well as the development of physiological, behavioral, and toxicological outcomes following alcohol exposure.
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影响因子：
2.3
作者：
MURPHY, JM;GATTO, GJ;LI, TK
通讯作者：
LI, TK
DOI：
10.1111/j.1530-0277.2010.01432.x
发表时间：
2011-05-01
影响因子：
3.2
作者：
Dick, Danielle M.;Aliev, Fazil;Rose, Richard J.
通讯作者：
Rose, Richard J.
DOI：
10.1097/01.alc.0000164381.67723.76
发表时间：
2005-05-01
期刊：
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
Harford, TC;Grant, BF;Chen, CM
通讯作者：
Chen, CM
DOI：
10.1111/j.1530-0277.2009.00955.x
发表时间：
2009-07-01
影响因子：
3.2
作者：
Oberlin, B. G.;Grahame, N. J.
通讯作者：
Grahame, N. J.
影响因子：
2.6
作者：
Grahame, NJ;Li, TK;Lumeng, L
通讯作者：
Lumeng, L