Genome-Wide Identification, Classification, Characterization, and Expression Analysis of the Wall-Associated Kinase Family during Fruit Development and under Wound Stress in Tomato (Solanum lycopersicum L.).
Genome-Wide Identification, Classification, Characterization, and Expression Analysis of the Wall-Associated Kinase Family during Fruit Development and under Wound Stress in Tomato (Solanum lycopersicum L.).
复制标题
番茄（Solanum lycopersicum L.）果实发育期间和伤口应激下壁相关激酶家族的全基因组鉴定、分类、表征和表达分析
作者：
Sun Z;Song Y;Chen D;Zang Y;Zhang Q;Yi Y;Qu G
细胞壁相关激酶（wall-associated kinase，WAK）和细胞壁相关激酶样（wall-associated kinase like，WAKL）是一类与细胞壁相关的受体样激酶，被认为是细胞外环境的传感器和细胞内信号的触发器。然而，这些蛋白质尚未在番茄（Solanum lycopersicum L.）中进行全面分析。在这项研究中，11个SlWAK和18个SlWAKL基因在12条染色体中的9条中不均匀分布。GUB-WAK-bind（壁相关受体激酶半乳糖醛酸聚糖结合）和表皮生长因子（EGF）结构域更常出现在SlWAK蛋白中。然而，更多的S1 WAKL（壁相关激酶样）具有WAK-asphalt（壁相关受体激酶C-末端）结构域。基于它们的系统发育关系，29个SlWAK-RLKs（壁相关激酶受体样激酶）被聚类为3个不同的类别，类似于拟南芥中的那些。各组内基因的保守基序高度相似。在这29个基因的启动子区的顺式元件被发现主要响应茉莉酸甲酯（MeJA），脱落酸（阿坝），水杨酸（SA），厌氧，光，伤口，和MYB转录因子。公开的番茄基因组RNA-seq数据表明，多种SlWAK-RLK在果实发育和成熟阶段表现出不同的表达模式，如SlWAK 4、SlWAKL 11、SlWAKL 9、SlWAKL 15、SlWAKL 14和SlWAKL 1，它们的RPKM（Reads Per Kilo bases per Million reads）值在果实膨大期不断增加，并随着果实成熟而降低。在番茄叶中，我们的RNA-seq数据显示，在机械创伤下，9种SlWAK-RLK转录物（SlWAK 3、SlWAK 4、SlWAK 10、SlWAKL 1、SlWAKL 2、SlWAKL 3、SlWAKL 5、SlWAKL 14和SlWAKL 18）被显著诱导（p < 0.001），并且3种转录物（SlWAK 2、SlWAK 5和SlWAKL 15）被显著抑制（p < 0.001）。SlWAKL 1和SlWAKL 6的qRT-PCR（定量逆转录聚合酶链反应）验证了这些结果。
The wall-associated kinase (WAK) and wall-associated kinase like (WAKL) is a subfamily of receptor-like kinases associated with the cell wall, which have been suggested as sensors of the extracellular environment and triggers of intracellular signals. However, these proteins have not yet been comprehensively analyzed in tomato (Solanum lycopersicum L.). In this study, 11 SlWAK and 18 SlWAKL genes were identified in an uneven distribution in 9 of 12 chromosomes. GUB-WAK-bind (wall-associated receptor kinase galacturonan-binding) and epidermal growth factor (EGF) domains appear more often in SlWAK proteins. However, more SlWAKLs (wall-associated kinase like) have a WAK-assoc (wall-associated receptor kinase C-terminal) domain. Based on their phylogenetic relationships, 29 SlWAK-RLKs (wall associated kinase-receptor like kinases) were clustered into three distinct categories analogous to those in Arabidopsis thaliana. High similarities were found in conserved motifs of the genes within each group. Cis-elements in the promoter region of these 29 genes were found mainly in response to methyl jasmonate (MeJA), abscisic acid (ABA), salicylic acid (SA), anaerobic, light, wound, and MYB transcription factors. Public tomato genome RNA-seq data indicates that multiple SlWAK-RLKs showed different expression patterns under developmental and ripening stages of fruits, such as SlWAK4, SlWAKL11, SlWAKL9, SlWAKL15, SlWAKL14, and SlWAKL1, their RPKM (Reads Per Kilo bases per Million reads) value constantly increases during the fruit expansion period, and decreases as the fruit matures. In tomato leaves, our RNA-seq data showed that nine SlWAK-RLKs transcripts (SlWAK3, SlWAK4, SlWAK10, SlWAKL1, SlWAKL2, SlWAKL3, SlWAKL5, SlWAKL14, and SlWAKL18) were significantly induced (p < 0.001), and three transcripts (SlWAK2, SlWAK5, and SlWAKL15) were significantly inhibited (p < 0.001) under mechanical wounding. The qRT-PCR (Quantitative reverse transcription polymerase chain reaction) of SlWAKL1 and SlWAKL6 verify these results.
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影响因子：
6.9
作者：
Baldassarre V;Cabassi G;Spadafora ND;Aprile A;Müller CT;Rogers HJ;Ferrante A
通讯作者：
Ferrante A
影响因子：
7.2
作者：
He, ZH;He, DZ;Kohorn, BD
通讯作者：
Kohorn, BD
DOI：
10.1098/rstb.2012.0003
发表时间：
2012-09-19
影响因子：
6.3
作者：
Lehti-Shiu, Melissa D.;Shiu, Shin-Han
通讯作者：
Shiu, Shin-Han
影响因子：
6.4
作者：
Campos-Vargas, R;Saltveit, ME
通讯作者：
Saltveit, ME
影响因子：
11.6
作者：
Lally, D;Ingmire, P;He, ZH
通讯作者：
He, ZH