Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites.
Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites.
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DOI：
10.1371/journal.pntd.0002911
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发表时间：
2014-05
影响因子：
3.8
通讯作者：
Van Den Abbeele J
中科院分区：
文献类型：
--
作者：
Caljon G;Duguma R;De Deken R;Schauvliege S;Gasthuys F;Duchateau L;Van Den Abbeele J
采采蝇是一种专职的食血昆虫，它传播非洲锥虫，导致人类昏睡病和家畜纳格纳。采采子唾液蛋白质组包含一个高度免疫原性的内切酶样蛋白家族。在这项研究中，Tsal1的一个重组版本(RTsal1)在间接ELISA中被用来量化与总舌蝇唾液的接触，从而量化采采蝇叮咬暴露。小鼠和猪被实验性地暴露于不同的革兰氏杆菌暴露方案中，随后对针对采采蝇总唾液和rTsal1的特异性抗体反应进行了长期跟踪。在小鼠中，一次采采蝇叮咬足以诱导可检测到的免疫球蛋白抗体反应，估计半衰期为36-40天。首次暴露后一年多可以检测到特异性抗体反应，咬一口就足以增强抗唾液免疫。此外，从采采烟暴露的猪身上收集的血浆显示，抗rTsal1和抗唾液免疫球蛋白水平增加，这与暴露强度有关。检测抗rTsal1抗体和抗唾液反应之间有很强的相关性。在两个受试动物模型中，ELISA检测性能和实验室内重复性都是足够的。研究了不同种类的粘虫(Glossina morsitans，G.m.morsitans，G.pallidipe，G.palpalis冈比亚和G.fuscipes)和其他食血昆虫(Stomoxys calcitrans和Tabanus药)对小鼠免疫球蛋白的交叉反应。这项研究阐明了来自变形杆菌的rTsal1作为接触广泛的舌状虫物种的敏感生物标记物的潜在用途。我们认为，抗rTsal1IgS的检测可能是采采蝇存在的一个有前景的血清学指标，将是监测采采蝇控制努力对非洲大陆影响的有价值的工具。嗜血性疾病媒介的唾液蛋白是暴露的潜在生物标记物，可用于补充昆虫学调查的血清学检测。我们证明，草原采采蝇(Glossina Morsitans Morsitans)高免疫原性Tsal1蛋白的重组版本是检测与采采蝇接触的敏感免疫探针。将小鼠和猪暴露于不同方案的采采蝇叮咬，结合血清学检测表明，rTsal1是一种敏感的指标，可以区分动物暴露的不同程度。采采集诱导的抗体持续时间相对较长，并在再次暴露时观察到有效的免疫增强。重组Tsal1是检测与不同采采蝇物种接触的一个很有前途的候选者，这将使在非洲大陆不同地区筛选接触采采蝇的种群或牛群成为可能。这种暴露指示器可能是一个有价值的工具，可以监测媒介控制程序的影响，并检测采采蝇对已清除区域的再次入侵。
Tsetse flies are obligate blood-feeding insects that transmit African trypanosomes responsible for human sleeping sickness and nagana in livestock. The tsetse salivary proteome contains a highly immunogenic family of the endonuclease-like Tsal proteins. In this study, a recombinant version of Tsal1 (rTsal1) was evaluated in an indirect ELISA to quantify the contact with total Glossina morsitans morsitans saliva, and thus the tsetse fly bite exposure. Mice and pigs were experimentally exposed to different G. m. morsitans exposure regimens, followed by a long-term follow-up of the specific antibody responses against total tsetse fly saliva and rTsal1. In mice, a single tsetse fly bite was sufficient to induce detectable IgG antibody responses with an estimated half-life of 36–40 days. Specific antibody responses could be detected for more than a year after initial exposure, and a single bite was sufficient to boost anti-saliva immunity. Also, plasmas collected from tsetse-exposed pigs displayed increased anti-rTsal1 and anti-saliva IgG levels that correlated with the exposure intensity. A strong correlation between the detection of anti-rTsal1 and anti-saliva responses was recorded. The ELISA test performance and intra-laboratory repeatability was adequate in the two tested animal models. Cross-reactivity of the mouse IgGs induced by exposure to different Glossina species (G. m. morsitans, G. pallidipes, G. palpalis gambiensis and G. fuscipes) and other hematophagous insects (Stomoxys calcitrans and Tabanus yao) was evaluated. This study illustrates the potential use of rTsal1 from G. m. morsitans as a sensitive biomarker of exposure to a broad range of Glossina species. We propose that the detection of anti-rTsal1 IgGs could be a promising serological indicator of tsetse fly presence that will be a valuable tool to monitor the impact of tsetse control efforts on the African continent. Salivary proteins of hematophagous disease vectors represent potential biomarkers of exposure and could be used in serological assays that are complementary to entomological surveys. We illustrate that a recombinant version of the highly immunogenic Tsal1 protein of the savannah tsetse fly (Glossina morsitans morsitans) is a sensitive immunological probe to detect contact with tsetse flies. Experimental exposure of mice and pigs to different regimens of tsetse fly bites combined with serological testing revealed that rTsal1 is a sensitive indicator that can differentiate the various degrees of exposure of animals. Tsetse-induced antibodies persisted relatively long, and an efficient boosting of immunity was observed upon re-exposure. Recombinant Tsal1 is a promising candidate to detect contact with various tsetse species, which would enable screening of populations or herds for exposure to tsetse flies in various areas on the African continent. This exposure indicator could be a valuable tool to monitor the impact of vector control programs and to detect re-invasion of cleared areas by tsetse flies.
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影响因子：
3
作者：
Poinsignon A;Cornelie S;Ba F;Boulanger D;Sow C;Rossignol M;Sokhna C;Cisse B;Simondon F;Remoue F
通讯作者：
Remoue F
影响因子：
3
作者：
Ali ZM;Bakli M;Fontaine A;Bakkali N;Vu Hai V;Audebert S;Boublik Y;Pagès F;Remoué F;Rogier C;Fraisier C;Almeras L
通讯作者：
Almeras L
影响因子：
3.8
作者：
Ndille, Emmanuel Elanga;Doucoure, Souleymane;Remoue, Franck
通讯作者：
Remoue, Franck
影响因子：
3.1
作者：
Caljon, Guy;Van Den Abbeele, Jan;Magez, Stefan
通讯作者：
Magez, Stefan
DOI：
10.4269/ajtmh.2010.09-0684
发表时间：
2010-07-01
影响因子：
3.3
作者：
Drame, Papa M.;Poinsignon, Anne;Remoue, Franck
通讯作者：
Remoue, Franck