C-terminals in the mouse branchiomotor nuclei originate from the magnocellular reticular formation.
C-terminals in the mouse branchiomotor nuclei originate from the magnocellular reticular formation.
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DOI：
10.1016/j.neulet.2013.05.063
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发表时间：
2013-08-26
影响因子：
2.5
通讯作者：
Kobayashi Y
中科院分区：
文献类型：
--
作者：
Matsui T;Hongo Y;Haizuka Y;Kaida K;Matsumura G;Martin DM;Kobayashi Y
被称为“c -末端”的大胆碱能突触钮扣与运动神经元接触并调节其兴奋性。脊髓体细胞运动核的c -末端起源于表达转录因子Pitx2的VII和X层胆碱能中间神经元。颅运动核包含另一种类型的运动神经元：支运动神经元。尽管支运动神经元接收到大量的c -末端投射，但这些c -末端的神经来源尚不清楚。在本研究中，我们首先检查了胆碱能神经元是否在成年小鼠脑干的网状结构中表达Pitx2，就像在脊髓中一样。虽然pitx2阳性的胆碱能神经元存在于大细胞网状结构和尾髓中央管周围区域，但没有一个在脑干被盖中更明显地存在。接下来，我们利用生物素化右旋糖酐胺（BDA）探索了支运动核中c -末端的起源。将BDA注射到髓质和脑桥的大细胞网状结构中，可在鳃裂运动核（歧义核、面核和三叉神经运动核）中标记许多c -末端。我们的研究结果表明，在支运动核中，c -末端的起源是大细胞网状结构中的胆碱能神经元，不仅在尾侧延髓中，而且在脑干的吻侧水平，缺乏pitx2阳性神经元。
Large cholinergic synaptic boutons called "C-terminals" contact motoneurons and regulate their excitability. C-terminals in the spinal somatic motor nuclei originate from cholinergic interneurons in laminae VII and X that express a transcription factor Pitx2. Cranial motor nuclei contain another type of motoneuron: branchiomotor neurons. Although branchiomotor neurons receive abundant C-terminal projections, the neural source of these C-terminals remains unknown. In the present study, we first examined whether cholinergic neurons express Pitx2 in the reticular formation of the adult mouse brainstem, as in the spinal cord. Although Pitx2-positive cholinergic neurons were observed in the magnocellular reticular formation and region around the central canal in the caudal medulla, none was present more rostrally in the brainstem tegmentum. We next explored the origin of C-terminals in the branchiomotor nuclei by using biotinylated dextran amine (BDA). BDA injections into the magnocellular reticular formation of the medulla and pons resulted in the labeling of numerous C-terminals in the branchiomotor nuclei: the ambiguous, facial, and trigeminal motor nuclei. Our results revealed that the origins of C-terminals in the branchiomotor nuclei are cholinergic neurons in the magnocellular reticular formation not only in the caudal medulla, but also at more rostral levels of the brainstem, which lacks Pitx2-positive neurons.
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影响因子：
3.5
作者：
Waite, Mindy R.;Skaggs, Kaia;Kaviany, Parisa;Skidmore, Jennifer M.;Causeret, Frederic;Martin, James F.;Martin, Donna M.
通讯作者：
Martin, Donna M.
影响因子：
2.7
作者：
Martin, DM;Skidmore, JM;Camper, SA
通讯作者：
Camper, SA
影响因子：
3.3
作者：
LI, W;OCHALSKI, PAY;NAGY, JI
通讯作者：
NAGY, JI
影响因子：
2.5
作者：
JONES, BE
通讯作者：
JONES, BE
DOI：
10.1007/bf00482985
发表时间：
1986-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
DAVIDOFF, MS;IRINTCHEV, AP
通讯作者：
IRINTCHEV, AP