Honey bee (Apis mellifera) gut microbiota promotes host endogenous detoxification capability via regulation of P450 gene expression in the digestive tract-猫眼课题宝

蜜蜂 (Apis mellifera) 肠道微生物群通过调节消化道 P450 基因表达促进宿主内源性解毒能力

DOI：

10.1111/1751-7915.13579

发表时间：

2020-04

期刊：

MICROBIAL BIOTECHNOLOGY

影响因子：

5.7

通讯作者：

中科院分区：

工程技术2区

文献类型：

作者：

Yuqi Wu;Yufei Zheng;Yanan Chen;Shuai Wang;Yanping Chen;Fuliang Hu;Huoqing Zheng

越来越多的研究表明，昆虫肠道微生物群与杀虫剂耐药性之间存在密切关系。然而，蜜蜂肠道菌群对宿主解毒能力的贡献尚未得到研究。为了解决这一问题，我们比较了肠道菌群缺陷（GD）工人和常规肠道菌群（CV）工人之间细胞色素p4500s （p4500s）基因的表达，并比较了噻虫啉或tau‐氟valinate处理GD和CV工人的死亡率和农药残留水平。我们的研究结果表明，肠道菌群促进P450酶在中肠的表达，GD工人的死亡率和农药残留水平显著高于CV工人。进一步比较四环素处理的工人和未处理的工人表明，抗生素诱导的肠道生态失调导致p450在中肠的表达减弱。抗生素和杀虫剂的共同处理导致蜜蜂存活率降低，农药残留显著增加。综上所述，我们的研究结果表明，蜜蜂肠道共生体可能通过改变宿主的外源解毒途径来促进蜜蜂的健康，并揭示了抗生素对蜜蜂解毒能力和健康的潜在负面影响。

There is growing number of studies demonstrating a close relationship between insect gut microbiota and insecticide resistance. However, the contribution of the honey bee gut microbiota to host detoxification ability has yet to be investigated. In order to address this question, we compared the expression of cytochrome P450s (P450s) genes between gut microbiota deficient (GD) workers and conventional gut community (CV) workers and compared the mortality rates and the pesticide residue levels of GD and CV workers treated with thiacloprid or tau‐fluvalinate. Our results showed that gut microbiota promotes the expression of P450 enzymes in the midgut, and the mortality rate and pesticide residue levels of GD workers are significantly higher than those of CV workers. Further comparisons between tetracycline‐treated workers and untreated workers demonstrated that antibiotic‐induced gut dysbiosis leads to attenuated expression of P450s in the midgut. The co‐treatment of antibiotics and pesticides leads to reduced survival rate and a significantly higher amount of pesticide residues in honey bees. Taken together, our results demonstrated that honey bee gut symbiont could contribute to bee health through the modification of the host xenobiotics detoxification pathways and revealed a potential negative impact of antibiotics to honey bee detoxification ability and health.