Inactivation of nucleus reuniens impairs spatial working memory and behavioral flexibility in the rat.
Inactivation of nucleus reuniens impairs spatial working memory and behavioral flexibility in the rat.
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DOI：
10.1002/hipo.22831
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发表时间：
2018-04
期刊：
影响因子：
3.5
通讯作者：
Vertes RP
中科院分区：
文献类型：
--
作者：
Viena TD;Linley SB;Vertes RP
海马结构(HF)和内侧前额叶皮质(MPFC)在空间工作记忆(SWM)中起着重要作用。丘脑腹侧中线核(RE)是连接HF和mPFC的重要解剖学纽带，因此与SWM功能密切相关。然而，关于RE在该回路介导的其他行为中的作用，人们知之甚少。在这项研究中，我们研究了RE在空间工作记忆和执行功能中的作用，在RE被蝇草酚或普鲁卡因可逆失活后。在大鼠体内植入靶向RE的留置导管，并进行延迟不匹配训练，以完成空间交替T迷宫任务。对于任务，样本运行和选择运行由中等或较长的延迟(30、60和120秒)分隔。根据渐近表现，大鼠在注射药物或赋形剂后进行测试。在所有延迟时，在RE中注入蝇草酚都会损害SWM，而普鲁卡因仅在最长延迟时损害表现。此外，用蝇草酚灭活RE导致了Win-Shift策略的失败以及严重的空间坚持，即在纠正试验中，尽管没有奖励，大鼠仍不断地重新进入错误的手臂。这表明了行为灵活性和反应策略的显著变化。这些结果加强了团结核在认知和执行功能中作为连接海马区和前额叶皮质的关键纽带的作用，并提示团结核可能成为治疗精神分裂症、注意缺陷多动障碍、成瘾和强迫症等中枢神经系统疾病的潜在靶点，这些疾病的症状是由海马-前额叶功能障碍定义的。
The hippocampal formation (HF) and medial prefrontal cortex (mPFC) play critical roles in spatial working memory (SWM). The nucleus reuniens (RE) of the ventral midline thalamus is an important anatomical link between the HF and mPFC, and as such is crucially involved SWM functions that recruit both structures. Little is known, however, regarding the role of RE in other behaviors mediated by this circuit. In the present study, we examined the role of RE in spatial working memory and executive functioning following reversible inactivation of RE with either muscimol or procaine. Rats were implanted with an indwelling cannula targeting RE and trained in a delayed nonmatch to sample spatial alternation T-maze task. For the task, sample and choice runs were separated by moderate or long delays (30, 60, and 120s). Following asymptotic performance, rats were tested following infusions of drug or vehicle. Muscimol infused into RE impaired SWM at all delays, whereby procaine only impaired performance at the longest delays. Furthermore, RE inactivation with muscimol produced a failure in win-shift strategy as well as severe spatial perseveration, whereby rats persistently made re-entries into incorrect arms during correction trials, despite the absence of reward. This demonstrated marked changes in behavioral flexibility and response strategy. These results strengthen the role of nucleus reuniens as a pivotal link between hippocampus and prefrontal cortex in cognitive and executive functions, and suggest that nucleus reuniens may be a potential target in the treatment of CNS disorders such as schizophrenia, attention deficit hyperactivity disorder, addiction, and obsessive-compulsive disorder, whose symptoms are defined by hippocampal-prefrontal dysfunctions.
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影响因子：
3
作者：
Cassaday HJ;Nelson AJ;Pezze MA
通讯作者：
Pezze MA
影响因子：
3.5
作者：
Ainge, James A.;van der Meer, Matthijs A. A.;Wood, Emma R.
通讯作者：
Wood, Emma R.
影响因子：
3.4
作者：
Abela, Andrew R.;Chudasama, Yogita
通讯作者：
Chudasama, Yogita
影响因子：
2.7
作者：
Chudasama, Y;Passetti, F;Robbins, TW
通讯作者：
Robbins, TW
影响因子：
2.7
作者：
DEBRUIN, JPC;VANOYEN, HGM;VANDEPOLL, N
通讯作者：
VANDEPOLL, N