Silver-nanoparticles-modified biomaterial surface resistant to staphylococcus: new insight into the antimicrobial action of silver.
Silver-nanoparticles-modified biomaterial surface resistant to staphylococcus: new insight into the antimicrobial action of silver.
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银纳米粒子修饰的生物材料表面可抵抗葡萄球菌：对银抗菌作用的新见解
DOI：
10.1038/srep32699
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发表时间：
2016-09-07
影响因子：
4.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Wang J;Li J;Guo G;Wang Q;Tang J;Zhao Y;Qin H;Wahafu T;Shen H;Liu X;Zhang X
钛种植体在临床上应用广泛，但术后种植体感染仍是一个潜在的严重并发症。本研究的目的是研究纳米银（Ag）功能化的钛表面对流行性葡萄球菌的抗菌活性从生物膜相关基因的调控的角度和细菌-细胞共培养研究的基础上。为了实现这一目标，两个代表性的流行性葡萄球菌菌株，表皮葡萄球菌（S。表皮葡萄球菌，RP 62 A）和金黄色葡萄球菌（S. aureus，USA 300），并且发现Ag纳米颗粒修饰的Ti表面可以调节生物膜相关基因（对于S. fnbA和fnbB对S.金黄色葡萄球菌）以抑制细菌粘附和生物膜形成。此外，一项新的细菌-成纤维细胞共培养研究表明，在这种表面上掺入Ag纳米颗粒可以帮助哺乳动物细胞比葡萄球菌更成功地存活、粘附和传播。因此，改性表面被证明具有良好的抗感染能力，对固着细菌和寄生细菌之间的协同作用的银纳米粒子和离子释放。这项工作提供了新的见解银纳米粒子功能化的钛表面的抗菌作用和机制，具有杀菌和细胞辅助能力，并铺平了道路，以更好地满足临床需求。
Titanium implants are widely used clinically, but postoperative implant infection remains a potential severe complication. The purpose of this study was to investigate the antibacterial activity of nano-silver(Ag)-functionalized Ti surfaces against epidemic Staphylococcus from the perspective of the regulation of biofilm-related genes and based on a bacteria-cell co-culture study. To achieve this goal, two representative epidemic Staphylococcus strains, Staphylococcus epidermidis (S. epidermidis, RP62A) and Staphylococcus aureus (S. aureus, USA 300), were used, and it was found that an Ag-nanoparticle-modified Ti surface could regulate the expression levels of biofilm-related genes (icaA and icaR for S. epidermidis; fnbA and fnbB for S. aureus) to inhibit bacterial adhesion and biofilm formation. Moreover, a novel bacteria-fibroblast co-culture study revealed that the incorporation of Ag nanoparticles on such a surface can help mammalian cells to survive, adhere and spread more successfully than Staphylococcus. Therefore, the modified surface was demonstrated to possess a good anti-infective capability against both sessile bacteria and planktonic bacteria through synergy between the effects of Ag nanoparticles and ion release. This work provides new insight into the antimicrobial action and mechanism of Ag-nanoparticle-functionalized Ti surfaces with bacteria-killing and cell-assisting capabilities and paves the way towards better satisfying the clinical needs.
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影响因子：
14
作者：
Antoci, Valentin, Jr.;Adams, Christopher S.;Parvizi, Javad;Davidson, Helen M.;Composto, Russell J.;Freeman, Theresa A.;Wickstrom, Eric;Ducheyne, Paul;Jungkind, Donald;Shapiro, Irving M.;Hickok, Noreen J.
通讯作者：
Hickok, Noreen J.
影响因子：
14
作者：
de Avila ED;Lima BP;Sekiya T;Torii Y;Ogawa T;Shi W;Lux R
通讯作者：
Lux R
影响因子：
12.8
作者：
Choi, Okkyoung;Deng, Kathy Kanjun;Hu, Zhiqiang
通讯作者：
Hu, Zhiqiang
影响因子：
4.9
作者：
Arciola, CR;Campoccia, D;Montanaro, L
通讯作者：
Montanaro, L
影响因子：
1.7
作者：
Costerton, JW;Montanaro, L;Arciola, CR
通讯作者：
Arciola, CR