Self-assembled gel tubes, filaments and 3D-printing with in situ metal nanoparticle formation and enhanced stem cell growth.
Self-assembled gel tubes, filaments and 3D-printing with in situ metal nanoparticle formation and enhanced stem cell growth.
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自组装凝胶管、细丝以及具备原位金属纳米颗粒生成和促进干细胞生长特性的3D打印技术。
DOI：
10.1039/d1sc06062g
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发表时间：
2022-02-16
期刊：
影响因子：
8.4
通讯作者：
Smith DK
中科院分区：
文献类型：
--
作者：
Piras CC;Kay AG;Genever PG;Fitremann J;Smith DK
本文报道了用低分子质量凝胶剂(LMWG)制备自组装人工管状和丝状体系的简单策略。在第一种策略中，将基于二亚甲基山梨醇的LMWG DBS-CONHNH2与聚合物凝胶剂(PG)海藻酸钙相结合，制备了管状“核-壳”凝胶结构。在第二种方法中，通过“溶剂切换”方法在高浓度下湿法纺丝制备了单独基于DBS-CONHNH2的凝胶长丝。湿法纺纱中使用的浓度较高，因此不需要支持PG。此外，这可以扩展到3D打印方法，打印的LMWG对象在水中至少一周表现出极好的稳定性。LMWG保留了其独特的原位贵金属还原能力，当管子和细丝暴露在AuCl3溶液中时，可以在管子和细丝中生成Au纳米颗粒(AuNPs)。由于凝胶丝具有较高的DBS-CONHNH2负载，因此它们可以负载显著更多的AuNPs。对人骨髓间充质干细胞的细胞毒性和活性研究表明，负载AuNPs的DBS-CONHNH2和DBS-CONHNH2/藻酸盐杂化凝胶具有良好的生物相容性，AuNPs的存在促进了干细胞的代谢。综上所述，这些结果表明DBS-CONHNH2可以成形和3D打印，并在组织工程应用中具有相当大的潜力。简单的制造和3D打印方法被用来从自组装凝胶中产生管子和细丝，这些凝胶可以原位负载金纳米颗粒，从而产生促进干细胞增殖的凝胶。
This paper reports simple strategies to fabricate self-assembled artificial tubular and filamentous systems from a low molecular weight gelator (LMWG). In the first strategy, tubular ‘core–shell’ gel structures based on the dibenzylidenesorbitol-based LMWG DBS-CONHNH2 were made in combination with the polymer gelator (PG) calcium alginate. In the second approach, gel filaments based on DBS-CONHNH2 alone were prepared by wet spinning at elevated concentrations using a ‘solvent-switch’ approach. The higher concentrations used in wet-spinning prevent the need for a supporting PG. Furthermore, this can be extended into a 3D-printing method, with the printed LMWG objects showing excellent stability for at least a week in water. The LMWG retains its unique ability for in situ precious metal reduction, yielding Au nanoparticles (AuNPs) within the tubes and filaments when they are exposed to AuCl3 solutions. Since the gel filaments have a higher loading of DBS-CONHNH2, they can be loaded with significantly more AuNPs. Cytotoxicity and viability studies on human mesenchymal stem cells show that the DBS-CONHNH2 and DBS-CONHNH2/alginate hybrid gels loaded with AuNPs are biocompatible, with the presence of AuNPs enhancing stem cell metabolism. Taken together, these results indicate that DBS-CONHNH2 can be shaped and 3D-printed, and has considerable potential for use in tissue engineering applications. Simple fabrication and 3D-printing methods are used to generate tubes and filaments from self-assembled gels, which can be loaded in situ with gold nanoparticles, with the resulting gels encouraging stem cell proliferation.
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影响因子：
5.4
作者：
Bianchi E;Ruggeri M;Rossi S;Vigani B;Miele D;Bonferoni MC;Sandri G;Ferrari F
通讯作者：
Ferrari F
影响因子：
3
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影响因子：
5.7
作者：
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通讯作者：
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影响因子：
62.1
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通讯作者：
Xu B
影响因子：
11
作者：
Chalard, Anais;Mauduit, Morgane;Fitremann, Juliette
通讯作者：
Fitremann, Juliette