Cytogenetic analysis of Anopheles ovengensis revealed high structural divergence of chromosomes in the Anopheles nili group.

Cytogenetic analysis of Anopheles ovengensis revealed high structural divergence of chromosomes in the Anopheles nili group.
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DOI:
10.1016/j.meegid.2013.03.010
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发表时间:
2013-06
期刊:
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
影响因子:
--
通讯作者:
Sharakhov IV
Sharakhov IV
中科院分区:
其他
文献类型:
--
作者:
Sharakhova MV;Peery A;Antonio-Nkondjio C;Xia A;Ndo C;Awono-Ambene P;Simard F;Sharakhov IV

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细胞遗传学分析是了解一组密切相关的蚊子物种成员之间关系的一种信息丰富的经典方法。欧氏按蚊是一种新近发现的安氏按蚊。nili属,是非洲赤道森林重要的疟疾媒介之一。本研究对安属植物的多染色体进行了研究。并将其与安的多丝染色体进行了比较。nili。利用荧光原位杂交和染色体带型比较,我们确定了安的染色体臂之间的对应关系。欧文和安。nili。对这两个物种的染色体形态分析显示,它们的带型有一定的相似性。最广泛的重组发生在中心周围和间质异染色质中。安的染色体。它们由一个弥散色中心连接在一起,在臂2L和臂3R有两个大的骨髓间异染色质区域。相反,在An中未见色中心和肾间异染色质。nili染色体。手臂关联的比较分析表明,两组成员之间发生了全手臂易位。观察到的染色体结构的大量重组表明,染色体进化的速度很快。或两种属属内不同的分类群。
Cytogenetic analysis is an informative classical approach to understanding the relationships among members in a group of closely related species of mosquitoes. Anopheles ovengensis is a recently discovered species of the An. nili group and is one of the important malaria vectors in the African equatorial forest. This study characterized polytene chromosomes of An. ovengensis and compared them with polytene chromosomes of An. nili. Using fluorescent in situ hybridization and chromosome banding pattern comparison we have established correspondence between chromosomal arms of An. ovengensis and An. nili. Analysis of chromosome morphology in the two species revealed a limited similarity in the banding patterns. The most extensive reorganization occurs in pericentromeric and intercalary heterochromatin. Chromosomes of An. ovengensis are joined together by a diffuse chromocenter and they have two large regions of intercalary heterochromatin in arms 2L and 3R. In contrast, the chromocenter and intercalary heterochromatin are not seen in An. nili chromosomes. Comparative analysis of the arm association suggests the occurrence of a whole-arm translocation between the two members of the group. The observed, substantial reorganizations of chromosome structure implies either a rapid rate of chromosome evolution in the An. nili group, or that the two species belong to different taxonomic groups within subgenus Cellia.
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期刊: PloS one
影响因子: 3.7
作者:
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