Phosphorus deprivation affects composition and spatial distribution of membrane lipids in legume nodules.
Phosphorus deprivation affects composition and spatial distribution of membrane lipids in legume nodules.
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DOI：
10.1093/plphys/kiaa115
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发表时间：
2021-04-23
期刊：
影响因子：
7.4
通讯作者：
Dickstein R
中科院分区：
文献类型：
--
作者：
Dokwal D;Romsdahl TB;Kunz DA;Alonso AP;Dickstein R
在豆科植物中，豆科植物宿主和根瘤菌之间成功相互作用后，共生固氮 (N) (SNF) 发生在称为根瘤的特殊器官中。在根瘤中，固氮根瘤菌被共生体膜包围，通过共生体膜，细菌和宿主豆科植物之间发生营养物质和铵的交换。磷（P）是一种必需的常量营养素，固氮豆科植物比依靠矿物质氮生长的豆科植物对磷的需求更高。与之前的研究一样，在磷缺乏的情况下，与在磷充足条件下生长的植物相比，桶形医学（Medicago truncatula）植物的SNF活性受损，生长减慢，并且在叶、根和根瘤中积累较少的磷酸盐。使用电喷雾电离质谱法评估蒺藜藻组织中的膜脂质。研究发现，缺磷时半乳糖脂增加，磷脂 (PL) 减少，尤其是在叶子中。根部和根瘤中的 PL 损失较低。随后，使用基质辅助激光解吸/电离质谱成像来绘制磷充足和缺乏磷条件下结节中带正电荷的磷脂酰胆碱（PC）物质的分布空间图。我们的结果揭示了结核中几种 PC 物种的不均匀分布，而其他 PC 类别的均匀分布。在缺磷条件下，观察到一些 PC 物种分布发生变化。结果表明，特定的 PC 种类在不同的结节区域和细胞类型中可能具有不同的重要性，并且 P 胁迫期间的膜脂重塑在整个结节中并不均匀。 ESI-MS 和基质辅助激光解吸电离质谱成像揭示了缺磷条件下蒺藜苜蓿根瘤膜脂质组成和空间分布的变化。
In legumes, symbiotic nitrogen (N) fixation (SNF) occurs in specialized organs called nodules after successful interactions between legume hosts and rhizobia. In a nodule, N-fixing rhizobia are surrounded by symbiosome membranes, through which the exchange of nutrients and ammonium occurs between bacteria and the host legume. Phosphorus (P) is an essential macronutrient, and N2-fixing legumes have a higher requirement for P than legumes grown on mineral N. As in the previous studies, in P deficiency, barrel medic (Medicago truncatula) plants had impaired SNF activity, reduced growth, and accumulated less phosphate in leaves, roots, and nodules compared with the plants grown in P sufficient conditions. Membrane lipids in M. truncatula tissues were assessed using electrospray ionization–mass spectrometry. Galactolipids were found to increase in P deficiency, with declines in phospholipids (PL), especially in leaves. Lower PL losses were found in roots and nodules. Subsequently, matrix-assisted laser desorption/ionization–mass spectrometry imaging was used to spatially map the distribution of the positively charged phosphatidylcholine (PC) species in nodules in both P-replete and P-deficient conditions. Our results reveal heterogeneous distribution of several PC species in nodules, with homogeneous distribution of other PC classes. In P poor conditions, some PC species distributions were observed to change. The results suggest that specific PC species may be differentially important in diverse nodule zones and cell types, and that membrane lipid remodeling during P stress is not uniform across the nodule. ESI–MS and matrix-assisted laser desorption ionization–mass spectrometry imaging reveal alterations in Medicago truncatula nodules membrane lipid composition and spatial distribution in phosphorus deficiency.
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DOI：
10.1016/j.jasms.2009.01.017
发表时间：
2009-06
影响因子：
3.2
作者：
Chen R;Hui L;Sturm RM;Li L
通讯作者：
Li L
DOI：
10.1111/pce.13872
发表时间：
2021-01
期刊：
Plant, cell & environment
影响因子：
--
作者：
Ding N;Huertas R;Torres-Jerez I;Liu W;Watson B;Scheible WR;Udvardi M
通讯作者：
Udvardi M
DOI：
10.1016/0304-4165(66)90383-7
发表时间：
1966-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
DILWORTH, MJ
通讯作者：
DILWORTH, MJ
影响因子：
3.8
作者：
Devaiah, Shivakumar Pattada;Roth, Mary R.;Wang, Xuemin
通讯作者：
Wang, Xuemin
DOI：
10.1007/s13361-014-1010-0
发表时间：
2015-01
影响因子：
3.2
作者：
Gemperline, Erin;Jayaraman, Dhileepkumar;Maeda, Junko;Ane, Jean-Michel;Li, Lingjun
通讯作者：
Li, Lingjun