Integrated Microbiome and Metabolome Analysis Reveals a Positive Change in the Intestinal Environment of Myostatin Edited Large White Pigs.
Integrated Microbiome and Metabolome Analysis Reveals a Positive Change in the Intestinal Environment of Myostatin Edited Large White Pigs.
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综合微生物组和代谢组分析揭示肌肉生长抑制素编辑的大白猪肠道环境发生积极变化
DOI：
10.3389/fmicb.2021.628685
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发表时间：
2021
影响因子：
5.2
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Pei Y;Chen C;Mu Y;Yang Y;Feng Z;Li B;Li H;Li K
肌肉生长抑制素（MSTN）功能失活可以改变猪的瘦肉和脂肪含量的比例。虽然基因型和微生物组成都已知会影响宿主表型，但到目前为止，还没有系统的研究来检测MSTs单拷贝突变猪的肠道微生物组成和代谢组的变化。在这里，我们使用16 S rDNA测序和代谢组学分析来研究MSTK基因编辑如何影响大白色猪空肠和盲肠中微生物和代谢组学组成的变化。我们的研究结果表明，梭菌_sensu_stricto_1，双歧杆菌，毛螺菌科_UCG-007，梭菌_sensu_stricto_6，瘤胃球菌科_UCG-002，和瘤胃球菌科_UCG-004显着上调，而密螺旋体_2和T34_未分类的显着下调在空肠的梅利利亚猪。类似地，在蒙古猪的盲肠中，Phascolarctobacterium、Ruminiclostridium_9、Succinivibrio、Longibaculum和Stoquefichus显著上调，而Barnesiella显著下调。此外，代谢组学分析显示，空肠中参与嘌呤、鞘脂和色氨酸代谢的代谢物发生了显著变化，而盲肠中与甘油磷脂和嘧啶代谢相关的代谢物发生了变化。斯皮尔曼相关性分析进一步证明了微生物群落组成与代谢产物之间存在显著的相关性。我们的分析表明，MIBI编辑影响空肠和盲肠中代谢物和微生物菌株的组成，这可能为MIBI ±大白色猪的宿主提供更多可用的营养。
Myostatin (MSTN) functional inactivation can change the proportion of lean meat and fat content in pigs. While both genotype and microbial composition are known to affect the host phenotype, so far there has been no systematic study to detect the changes in the intestinal microbial composition and metabolome of MSTN single copy mutant pigs. Here, we used 16S rDNA sequencing and metabolome analysis to investigate how MSTN gene editing affects changes in the microbial and metabolome composition in the jejunum and the cecum of Large White pigs. Our results showed that Clostridium_sensu_stricto_1, Bifidobacterium, Lachnospiraceae_UCG-007, Clostridium_sensu_stricto_6, Ruminococcaceae_UCG-002, and Ruminococcaceae_UCG-004 were significantly upregulated; while Treponema_2 and T34_unclassified were significantly downregulated in the jejunum of MSTN pigs. Similarly, Phascolarctobacterium, Ruminiclostridium_9, Succinivibrio, Longibaculum, and Candidatus_Stoquefichus were significantly upregulated, while Barnesiella was significantly downregulated in the cecum of MSTN pigs. Moreover, metabolomics analysis showed significant changes in metabolites involved in purine, sphingolipid and tryptophan metabolism in the jejunum, while those associated with glycerophospholipid and pyrimidine metabolism were changed in the cecum. Spearman correlation analysis further demonstrated that there was a significant correlation between microflora composition and metabolites. Our analyses indicated the MSTN editing affects the composition of metabolites and microbial strains in the jejunum and the cecum, which might provide more useable nutrients for the host of MSTN± Large White pigs.
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影响因子：
4.6
作者：
Chen S;Wang J;Peng D;Li G;Chen J;Gu X
通讯作者：
Gu X
影响因子：
15.5
作者：
Lu D;Tiezzi F;Schillebeeckx C;McNulty NP;Schwab C;Shull C;Maltecca C
通讯作者：
Maltecca C
影响因子：
5.4
作者：
Hu, Shiwei;Xu, Yangli;Yang, Huicheng
通讯作者：
Yang, Huicheng
影响因子：
3.3
作者：
Mach, Nuria;Berri, Mustapha;Lepage, Patricia
通讯作者：
Lepage, Patricia
影响因子：
5.6
作者：
Hou, Dianzhi;Zhao, Qingyu;Shen, Qun
通讯作者：
Shen, Qun