Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells.
Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells.
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源自诱导多能干细胞的单层中心肌细胞的光学图。
作者：
光学标测是一种强大的成像技术，广泛用于分别使用电压敏感染料和Ca2+指示剂测量可兴奋组织中的膜电位变化和细胞内Ca2+变化。这一强大的工具已迅速成为心脏电生理学领域不可或缺的研究去极化波传播，估计电脉冲的传导速度，并测量心脏细胞和组织中的Ca2+动力学。此外，标测这些电生理参数对于理解心律失常机制是重要的。在这篇综述中，我们深入研究了心脏光学标测技术的基本原理及其应用时，应用于hiPSC衍生的心肌细胞，并讨论了相关的优势和挑战。我们还提供了光学标测数据的处理和分析的详细描述，这是心脏疾病和心律失常机制的提取和比较相关的电生理参数的研究中的关键一步。
Optical mapping is a powerful imaging technique widely adopted to measure membrane potential changes and intracellular Ca2+ variations in excitable tissues using voltage-sensitive dyes and Ca2+ indicators, respectively. This powerful tool has rapidly become indispensable in the field of cardiac electrophysiology for studying depolarization wave propagation, estimating the conduction velocity of electrical impulses, and measuring Ca2+ dynamics in cardiac cells and tissues. In addition, mapping these electrophysiological parameters is important for understanding cardiac arrhythmia mechanisms. In this review, we delve into the fundamentals of cardiac optical mapping technology and its applications when applied to hiPSC-derived cardiomyocytes and discuss related advantages and challenges. We also provide a detailed description of the processing and analysis of optical mapping data, which is a crucial step in the study of cardiac diseases and arrhythmia mechanisms for extracting and comparing relevant electrophysiological parameters.
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影响因子：
5.9
作者：
Riedel, Michael;Jou, Chuanchau J.;Lai, Shuping;Lux, Robert L.;Moreno, Alonso P.;Spitzer, Kenneth W.;Christians, Elizabeth;Tristani-Firouzi, Martin;Benjamin, Ivor J.
通讯作者：
Benjamin, Ivor J.
影响因子：
48
作者：
Burridge, Paul W.;Matsa, Elena;Shukla, Praveen;Lin, Ziliang C.;Churko, Jared M.;Ebert, Antje D.;Lan, Feng;Diecke, Sebastian;Huber, Bruno;Mordwinkin, Nicholas M.;Plews, Jordan R.;Abilez, Oscar J.;Cui, Bianxiao;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
DOI：
10.1007/978-3-319-17641-3_1
发表时间：
2015-01-01
期刊：
MEMBRANE POTENTIAL IMAGING IN THE NERVOUS SYSTEM AND HEART
影响因子：
--
作者：
Braubach，Oliver;Cohen，Lawrence B.;Choi，Yunsook
通讯作者：
Choi，Yunsook
影响因子：
4.5
作者：
Lee, Peter;Wang, Ken;Woods, Christopher E.;Yan, Ping;Kohl, Peter;Ewart, Paul;Loew, Leslie M.;Terrar, Derek A.;Bollensdorff, Christian
通讯作者：
Bollensdorff, Christian
DOI：
10.1007/978-1-0716-0668-1_10
发表时间：
2021
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Han L;Mich-Basso J;Kühn B
通讯作者：
Kühn B