Expression of Maf family proteins in glutamatergic neurons of the mouse olfactory bulb.
Expression of Maf family proteins in glutamatergic neurons of the mouse olfactory bulb.
复制标题
DOI：
10.1002/dneu.22859
复制
发表时间：
2022-01
影响因子：
3
通讯作者：
Imamura, Fumiaki
中科院分区：
文献类型：
--
作者：
Ito, Ayako;Imamura, Fumiaki
发育中的大脑神经元的命运在很大程度上取决于它们所表达的转录因子的组合。特别是，干细胞在分化过程中必须遵循不同的转录级联，以产生具有不同神经递质特性的神经元，如谷氨酸能神经元和gaba能神经元。在小鼠大脑皮层中，研究表明Maf家族大蛋白MafA、MafB和c-Maf调节特定类型gaba能中间神经元的发育，但在谷氨酸能神经元中不表达。本研究通过免疫组化方法检测了Maf家族大蛋白在发育中的小鼠嗅球中的表达，发现表达MafA和MafB的细胞群几乎相同，且大部分表达Tbr2。由于Tbr2在嗅球的谷氨酸能神经元中表达，我们进一步检测了MafA和MafB阳性细胞中谷氨酸能和gaba能神经元标志物的表达。结果表明，在嗅球中，MafA和MafB仅在谷氨酸能神经元中表达，而在gaba能神经元中不表达。我们还发现在嗅球中很少有细胞表达c-Maf。这些结果表明，与大脑皮层不同，MafA和/或MafB可能调节发育中的嗅球中谷氨酸能神经元的发育。本研究进一步加深了我们对嗅球中谷氨酸能神经元发育的认识，同时也揭示了皮层和嗅球虽然都是由端脑产生的，但它们产生不同性质的投射神经元和中间神经元的机制。
The fate of neurons in the developing brain is largely determined by the combination of transcription factors they express. In particular, stem cells must follow different transcriptional cascades during differentiation in order to generate neurons with different neurotransmitter properties, such as glutamatergic and GABAergic neurons. In the mouse cerebral cortex, it has been shown that large Maf family proteins, MafA, MafB and c-Maf, regulate the development of specific types of GABAergic interneurons but are not expressed in glutamatergic neurons. In this study, we examined the expression of large Maf family proteins in the developing mouse olfactory bulb by immunohistochemistry and found that the cell populations expressing MafA and MafB are almost identical, and most of them express Tbr2. Since Tbr2 is expressed in glutamatergic neurons in the olfactory bulb, we further examined the expression of glutamatergic and GABAergic neuronal markers in MafA and MafB positive cells. The results showed that in the olfactory bulb, MafA and MafB are expressed exclusively in glutamatergic neurons, but not in GABAergic neurons. We also found that few cells express c-Maf in the olfactory bulb. These results indicate that, unlike the cerebral cortex, MafA and/or MafB may regulate the development of glutamatergic neurons in the developing olfactory bulb. This study advances our knowledge about the development of glutamatergic neurons in the olfactory bub, and also provides insight into the mechanism by which the cortex and olfactory bulb, although both generated from the telencephalon, generate projection and interneurons with different properties.
登录
查看更多内容
影响因子：
3.7
作者：
Del Barrio, Marta Garcia;Bourane, Steeve;Goulding, Martyn
通讯作者：
Goulding, Martyn
影响因子：
2.5
作者：
Lepousez, Gabriel;Csaba, Zsolt;Viollet, Cecile
通讯作者：
Viollet, Cecile
影响因子：
16.2
作者：
Hevner, RF;Shi, LM;Rubenstein, JLR
通讯作者：
Rubenstein, JLR
影响因子：
10.9
作者：
Hang, Yan;Stein, Roland
通讯作者：
Stein, Roland
影响因子：
4.6
作者：
Chen YJ;Friedman BA;Ha C;Durinck S;Liu J;Rubenstein JL;Seshagiri S;Modrusan Z
通讯作者：
Modrusan Z