Intranasal immunization with a recombinant outer membrane protein H based Haemorrhagic septicemia vaccine in dairy calves.
Intranasal immunization with a recombinant outer membrane protein H based Haemorrhagic septicemia vaccine in dairy calves.
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DOI：
10.1292/jvms.17-0176
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发表时间：
2018-01-27
期刊：
影响因子：
--
通讯作者：
Sthitmatee N
中科院分区：
文献类型：
--
作者：
Muangthai K;Tankaew P;Varinrak T;Uthi R;Rojanasthien S;Sawada T;Sthitmatee N
出血性败血症是一种发病率和死亡率都很高的牛传染病。泰国的HS疫苗是一种油佐剂配方，很难管理。本研究旨在制定和评价经鼻接种HS疫苗对奶牛的保护作用。鼻腔疫苗是由多杀性巴氏杆菌M-1404(血清型B：2)的重组外膜蛋白H(ROmpH)50或100微克，以及10微克胞嘧啶-磷酸-鸟苷脱氧核苷酸作为粘膜佐剂制成的。每隔三周进行三次鼻腔免疫。免疫后的抗体用间接酶联免疫吸附试验检测，显示了有效地抑制补体介导的杀伤试验中的多杀性巴氏杆菌菌株的体外活性。鼻用疫苗诱导的血清免疫球蛋白和分泌型免疫球蛋白A水平均显著高于注射疫苗(P<；0.05)。在实验的第72天，用多杀性巴氏杆菌菌株M-1404进行攻击暴露。接种疫苗的小牛在挑战暴露后临床体征减少，这通常会导致疾病扩散。结论用CpG-ODN2007经鼻腔免疫小牛，可产生抗多杀性巴氏杆菌M-1404株rOmpH和全细胞抗原的血清和分泌性抗体。此外，它还能保护小牛免受人工感染多杀性巴氏杆菌的感染。
Haemorrhagic septicemia (HS) is a contagious disease in cattle with high morbidity and mortality rates. HS vaccine in Thailand is an oil-adjuvant formulation, and is difficult to administer. The present study aimed to formulate and evaluate the protection in dairy calves conferred by immunization with an in-house intranasal HS vaccine. The intranasal vaccine was formulated in a total volume of 500 µl containing either 50 or 100 µg of the recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain M-1404 (serovar B:2), and 10 µg of Cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) as a mucosal adjuvant. Intranasal immunizations were conducted three times at three-week intervals. The antibodies post-immunization were detected by indirect ELISA and demonstrated efficient in vitro activity in suppressing a P. multocida strain from the complement-mediated killing assay. An intranasal vaccine induced both the serum IgG and secretory IgA levels that were significantly higher than the level conferred by the parenteral vaccine (P<0.05). Challenge exposure was conducted with a P. multocida strain M-1404 at day 72 of the experiments. The immunized calves had reduced clinical signs after challenge exposure that would normally result in disease proliferation. We conclude that intranasal vaccination of calves with rOmpH with CpG-ODN 2007 stimulated serum and secretory antibodies to rOmpH and whole cells of P. multocida strain M-1404 antigen. Moreover, it would result in protection in calves against artificial P. multocida infection.
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4.4
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