Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes.
Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes.
复制标题
DOI：
10.1371/journal.pone.0068484
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Bjarnsholt T
中科院分区：
文献类型：
--
作者：
Jakobsen TH;Hansen MA;Jensen PØ;Hansen L;Riber L;Cockburn A;Kolpen M;Rønne Hansen C;Ridderberg W;Eickhardt S;Hansen M;Kerpedjiev P;Alhede M;Qvortrup K;Burmølle M;Moser C;Kühl M;Ciofu O;Givskov M;Sørensen SJ;Høiby N;Bjarnsholt T
木糖嗜铬杆菌是一种环境条件致病菌，感染着越来越多的免疫缺陷患者。在这项研究中，我们将一株临床分离的木霉菌株的基因组分析与其重要致病特征的表型研究相结合。我们提供了木糖酵母菌NH44784-1996基因组的完整组装，这是1996年从一例囊性纤维化患者中分离出来的。木霉NH44784-1996的基因组全长约为700万个碱基对，有6390个潜在的蛋白质编码序列。我们鉴定了使其成为一种机会人类病原体的几个特征，我们发现了参与厌氧生长的基因和编码粘附素多聚-β-1，6-N-乙酰-D-氨基葡萄糖生物膜的pGaABCD操纵子。此外，基因组还包含一系列抗生素耐药基因，编码外排泵系统和抗生素修饰酶。木糖酵母菌NH44784-1996的体外研究证实了其形成生物膜、通过反硝化进行厌氧生长以及对多种抗生素产生抗药性的基因组证据。我们的研究使我们能够进一步研究与木霉致病相关的重要基因的功能，从而提高我们对这种新出现的病原体的理解和治疗能力。
Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of A. xylosoxidans NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of A. xylosoxidans NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of A. xylosoxidans and thereby improves our understanding and ability to treat this emerging pathogen.
登录
查看更多内容
DOI：
10.1016/j.ijantimicag.2009.08.015
发表时间：
2010-01-01
影响因子：
10.8
作者：
Almuzara, Marisa;Limansky, Adriana;Vay, Carlos
通讯作者：
Vay, Carlos
影响因子：
56.9
作者：
FLEISCHMANN, RD;ADAMS, MD;VENTER, JC
通讯作者：
VENTER, JC
影响因子：
9.4
作者：
Ceri, H;Olson, ME;Buret, A
通讯作者：
Buret, A
影响因子：
12.3
作者：
Crossman LC;Gould VC;Dow JM;Vernikos GS;Okazaki A;Sebaihia M;Saunders D;Arrowsmith C;Carver T;Peters N;Adlem E;Kerhornou A;Lord A;Murphy L;Seeger K;Squares R;Rutter S;Quail MA;Rajandream MA;Harris D;Churcher C;Bentley SD;Parkhill J;Thomson NR;Avison MB
通讯作者：
Avison MB
影响因子：
5.2
作者：
De Baets, Frans;Schelstraete, Petra;Vaneechoutte, Mario
通讯作者：
Vaneechoutte, Mario