Antibacterial 3D bone scaffolds for tissue engineering application.
Antibacterial 3D bone scaffolds for tissue engineering application.
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DOI：
10.1002/jbm.b.34199
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发表时间：
2019-05
影响因子：
3.4
通讯作者：
Handa, Hitesh
中科院分区：
文献类型：
--
作者：
Pant, Jitendra;Sundaram, Jaya;Goudie, Marcus J.;Dieu Thao Nguyen;Handa, Hitesh
关键词：
开放性骨折不仅难以愈合，而且感染的风险也很高。每年由骨质疏松症引起的骨折病例约为900万例。内源性释放的一氧化氮（NO）已被证明在成骨分化中发挥作用，除了根除对各种病原体的感染。本研究以S-亚硝基-N-乙酰青霉胺（SNAP）为NO供体，制备了具有抗菌活性的NO释放三维骨支架。在制造过程中，将纳米羟基磷灰石（nHA）以nHA-淀粉-藻酸盐和nHA-淀粉-壳聚糖支架中10-50重量%的浓度范围添加到每个支架中。支架的力学强度随nHA浓度的增加而增加，其中nHA-淀粉-海藻酸钠质量分数为50%时，支架的承载能力最高，为203.95 ± 0.3N。发现50 wt % nHA-淀粉-藻酸盐支架的NO通量最初为0.50 ± 0.06 × 10−10 mol/min/mg，在生理条件下24 h后降至0.23 ± 0.02 × 10−10。结果，观察到革兰氏阳性细菌金黄色葡萄球菌的99.76% ± 0.33%减少和革兰氏阴性细菌铜绿假单胞菌的粘附活菌落的99.80% ± 0.62%减少，这是抗菌天然聚合物领域的重大进步。观察到表面形态和孔径适合于潜在的骨细胞生长。该材料对小鼠成纤维细胞无毒性反应。
Open bone fractures are not only difficult to heal but also are at a high risk of infections. Annual cases of fractures which result from osteoporosis amount to approximately 9 million. Endogenously released nitric oxide (NO) has been shown to play a role in osteogenic differentiation in addition to eradicating infection against a wide variety of pathogens. In the current work, antimicrobial NO releasing 3D bone scaffolds were fabricated using S-nitroso-N-acetyl-penicillamine (SNAP) as the NO donor. During fabrication, nano-hydroxyapatite (nHA) was added to each of the scaffolds in the concentration range of 10–50 wt % in nHA-starch-alginate and nHA-starch-chitosan scaffolds. The mechanical strength of the scaffolds increased proportionally to the concentration of nHA and 50 wt % nHA-starch-alginate possessed the highest load bearing capacity of 203.95 ± 0.3 N. The NO flux of the 50 wt % nHA-starch-alginate scaffolds was found to be 0.50 ± 0.06 × 10−10 mol/min/mg initially which reduced to 0.23 ± 0.02 × 10−10 over a 24 h period under physiological conditions. As a result, a 99.76% ± 0.33% reduction in a gram-positive bacterium, Staphylococcus aureus and a 99.80% ± 0.62% reduction in the adhered viable colonies of gram-negative bacterium, Pseudomonas aeruginosa were observed, which is a significant stride in the field of antibacterial natural polymers. The surface morphology and pore size were observed to be appropriate for the potential bone cell growth. The material showed no toxic response toward mouse fibroblast cells.
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影响因子：
4.9
作者：
Aronin, Caren E. Petrie;Sadik, Karim W.;Lay, Ann L.;Rion, Dave B.;Tholpady, Sunil S.;Ogle, Roy C.;Botchwey, Edward A.
通讯作者：
Botchwey, Edward A.
影响因子：
9.5
作者：
Grommersch BM;Pant J;Hopkins SP;Goudie MJ;Handa H
通讯作者：
Handa H
DOI：
10.1080/00914037.2016.1163570
发表时间：
2016
期刊：
International journal of polymeric materials
影响因子：
--
作者：
Goudie MJ;Brisbois EJ;Pant J;Thompson A;Potkay JA;Handa H
通讯作者：
Handa H
影响因子：
14
作者：
Cai, Q;Yang, JA;Wang, SG
通讯作者：
Wang, SG
影响因子：
4.1
作者：
Fox, SW;Chow, JWM
通讯作者：
Chow, JWM