Molecular Prevalence of Anaplasma marginale and Ehrlichia in Domestic Large Ruminants and Rhipicephalus (Boophilus) microplus Ticks From Southern Luzon, Philippines.
Molecular Prevalence of Anaplasma marginale and Ehrlichia in Domestic Large Ruminants and Rhipicephalus (Boophilus) microplus Ticks From Southern Luzon, Philippines.
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DOI：
10.3389/fvets.2021.746705
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发表时间：
2021
影响因子：
3.2
通讯作者：
Tanaka T
中科院分区：
文献类型：
--
作者：
Galay RL;Llaneta CR;Monreal MKFB;Armero AL 3rd;Baluyut ABD;Regino CMF;Sandalo KAC;Divina BP;Talactac MR;Tapawan LP;Mojares MCL;Alvarez CR;Mago ER;Encarnacion ND;Andoh M;Tanaka T
无形体病和埃立克体病是蜱传立克次体病，在全球畜牧业中造成重大经济损失。虽然已知牛无形体病是菲律宾的地方病，但流行病学数据不完整。此外，对该国的牛埃里希体病知之甚少。本研究采用PCR技术对菲律宾吕宋岛南部地区牛和布法罗中边缘无形体和埃立克体的感染情况进行了调查。对512头牛、108头布法罗和195头蜱的620份血液样品进行了巢式PCR，目的是扩增无形体科的groESL基因。阳性标本进一步进行巢式PCR和常规PCR扩增。marginale groEL基因和埃里希体dsbA基因。选择A。边缘阳性样品也进行靶向msp 5基因的巢式PCR。无论动物宿主如何，所获得的血液样本的总体患病率为无形体科51.9%，无形体科43%。边缘埃里希体1.1%。无布法罗埃立克体阳性。同时，15.9%、6.7%和2%的蜱样品（均经形态学鉴定为微小扇头蜱（牛蜱））对无形体科、A. marginale和埃里希体。序列分析表明，A.边缘msp 5扩增子显示，来自该区域的分离物与报道的A.来自其他国家的边缘。系统发育树显示该地区分离株聚为一类，与A.从其他国家分离的边缘。来自牛和蜱的埃里希体扩增子的序列与报道的米纳斯埃里希体分离株的相似性为97-100%。本研究表明A.在菲律宾吕宋岛发现的E.米纳斯在该国。
Anaplasmosis and ehrlichiosis are tick-borne rickettsial diseases that cause significant economic losses in the livestock industry worldwide. Although bovine anaplasmosis is known to be endemic in the Philippines, epidemiological data is fragmented. Moreover, little is known about bovine ehrlichiosis in the country. In this study, the prevalence of Anaplasma marginale and Ehrlichia in cattle and water buffalo from provinces in the southern part of Luzon, Philippines, was investigated through PCR. Blood samples from 620 animals comprised of 512 cattle and 108 water buffalo and 195 tick samples were subjected to nested PCR targeting the groESL gene of Anaplasmataceae. Positive samples were further subjected to another nested PCR and conventional PCR to amplify the A. marginale groEL gene and the Ehrlichia dsbA gene, respectively. Selected A. marginale-positive samples were also subjected to nested PCR targeting the msp5 gene. Regardless of the animal host, the overall prevalence in blood samples obtained was 51.9% for Anaplasmataceae, 43% for A. marginale, and 1.1% for Ehrlichia. No water buffalo were positive for Ehrlichia. Meanwhile, 15.9, 6.7, and 2% of the tick samples, all morphologically identified as Rhipicephalus (Boophilus) microplus, were positive for Anaplasmataceae, A. marginale, and Ehrlichia, respectively. Sequence analysis of selected A. marginale msp5 amplicons showed that the isolates from the region share 94–98% identity to reported A. marginale from other countries. The phylogenetic tree showed clustering of isolates in the region and a close relationship with A. marginale isolates from other countries. Sequences of Ehrlichia amplicons from cattle and ticks were 97–100% similar to reported Ehrlichia minasensis isolates. This study showed the high prevalence of A. marginale in Luzon, Philippines, and provided the first molecular evidence of E. minasensis in the country.
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DOI：
10.1099/ijsem.0.000895
发表时间：
2016-03-01
影响因子：
2.8
作者：
Cabezas-Cruz, Alejandro;Zweygarth, Erich;de la Fuente, Jose
通讯作者：
de la Fuente, Jose
影响因子：
2.6
作者：
Inokuma, H;Parola, P;Brouqui, P
通讯作者：
Brouqui, P
影响因子：
2.6
作者：
Tabara, Kenji;Arai, Satoru;Tsuji, Masayoshi
通讯作者：
Tsuji, Masayoshi
影响因子：
1.1
作者：
Bock, RE;deVos, AJ;McLellan, DJ
通讯作者：
McLellan, DJ
影响因子：
3.7
作者：
Hailemariam Z;Krücken J;Baumann M;Ahmed JS;Clausen PH;Nijhof AM
通讯作者：
Nijhof AM