Investigation of Cell Adhesion and Cell Viability of the Endothelial and Fibroblast Cells on Electrospun PCL, PLGA and Coaxial Scaffolds for Production of Tissue Engineered Blood Vessel.
Investigation of Cell Adhesion and Cell Viability of the Endothelial and Fibroblast Cells on Electrospun PCL, PLGA and Coaxial Scaffolds for Production of Tissue Engineered Blood Vessel.
复制标题
在电纺PCL，PLGA和同轴支架上，研究细胞粘附和细胞的细胞粘附和细胞活力，用于生产组织工程血管。
DOI：
10.3390/jfb13040282
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发表时间：
2022-12-08
影响因子：
4.8
通讯作者：
Sefat, Farshid
中科院分区：
文献类型：
--
作者：
Bazgir, Morteza;Saeinasab, Morvarid;Zhang, Wei;Zhang, Ximu;Tsui, Ka Min;Sarvestani, Abolfazl Maasoumi;Nawaz, Subhaan;Coates, Phil;Youseffi, Mansour;Elies, Jacobo;Sefat, Farshid
关键词：
人工支架的内皮化被认为是提高血管移植效率的有效策略。本研究旨在比较三种不同的生物可降解纤维支架的生物物理/生物相容性：单独的聚（ε-己内酯）（PCL）、单独的聚乳酸-乙醇酸（PLGA）（均使用 Spraybase® 静电纺丝机加工）以及纤维芯和鞘分别由 PCL 和 PLGA 制成的同轴支架。通过扫描电子显微镜评估支架结构形态，并使用拉伸测试来研究支架随时间的抗拉力。使用人脐静脉内皮细胞 (HUVEC) 和人血管成纤维细胞 (HVF) 进行生物相容性研究，分别通过细胞毒性测定和共聚焦显微镜评估细胞活力（以及 4 天期间的细胞增殖）和细胞对支架的粘附力。我们的结果表明，所有可生物降解的聚合物支架都是粘附和促进 HUVEC 和 HVF 细胞增殖的可靠宿主。特别是，与不存在聚合物的对照相比，PLGA 膜具有更好的粘附性并增强细胞增殖。此外，我们在这里证明这些可生物降解的膜具有改善的机械性能，可以构建潜在的组织工程血管移植物。
Endothelialization of artificial scaffolds is considered an effective strategy for increasing the efficiency of vascular transplantation. This study aimed to compare the biophysical/biocompatible properties of three different biodegradable fibrous scaffolds: Poly (ɛ-caprolactone) (PCL) alone, Poly Lactic-co-Glycolic Acid (PLGA) alone (both processed using Spraybase® electrospinning machine), and Coaxial scaffold where the fiber core and sheath was made of PCL and PLGA, respectively. Scaffold structural morphology was assessed by scanning electron microscope and tensile testing was used to investigate the scaffold tension resistance over time. Biocompatibility studies were carried out with human umbilical vein endothelial cells (HUVEC) and human vascular fibroblasts (HVF) for which cell viability (and cell proliferation over a 4-day period) and cell adhesion to the scaffolds were assessed by cytotoxicity assays and confocal microscopy, respectively. Our results showed that all biodegradable polymeric scaffolds are a reliable host to adhere and promote proliferation in HUVEC and HVF cells. In particular, PLGA membranes performed much better adhesion and enhanced cell proliferation compared to control in the absence of polymers. In addition, we demonstrate here that these biodegradable membranes present improved mechanical properties to construct potential tissue-engineered vascular graft.
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影响因子：
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作者：
Kwak S;Haider A;Gupta KC;Kim S;Kang IK
通讯作者：
Kang IK
影响因子：
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Pillay, Viness
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Homann, HH
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通讯作者：
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