CLE14/CLE20 peptides may interact with CLAVATA2/CORYNE receptor-like kinases to irreversibly inhibit cell division in the root meristem of Arabidopsis.
CLE14/CLE20 peptides may interact with CLAVATA2/CORYNE receptor-like kinases to irreversibly inhibit cell division in the root meristem of Arabidopsis.
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DOI：
10.1007/s00425-010-1236-4
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发表时间：
2010-10
期刊：
影响因子：
4.3
通讯作者：
Feldman LJ
中科院分区：
文献类型：
--
作者：
Meng L;Feldman LJ
为了了解CLAVATA（CLV）复合物的相互作用性质，我们预测了CLV 3/ESR相关（CLE）肽及其潜在受体蛋白/激酶的胞外域的三维结构，以及这些分子的对接模型。结果表明，CLV 1的胞外结构域可以形成同源二聚体，并且12-/13-氨基酸CLV 3肽适合CLV 1二聚体的结合裂缝。我们的研究结果还表明，CORYNE（CRN），最近确定的受体样激酶的受体结构域，紧密结合CLV 2的胞外域，这可能会导致与CLV 1对接的可能性增加。此外，我们的对接模型揭示了两个CRN-CLV 2胞外域异二聚体能够形成四聚体受体复合物。CLV 3、CLE 14、CLE 19和CLE 20的肽也能够结合潜在的CLV 2-CRN异二聚体或异四聚体复合物。使用细胞分裂报告线，我们发现，合成的12个氨基酸的CLE 14和CLE 20肽抑制，不可逆地，根生长，通过降低细胞分裂率在根顶端分生组织，导致短根表型。有趣的是，我们观察到外源性细胞分裂素的应用可以部分挽救由植物中的CLE 14或CLE 20的过表达诱导的短根表型。然而，细胞分裂素治疗不能挽救由外源性应用合成的CLE 14/CLE 20肽引起的短根表型，这表明需要仅在活植物中提供的条件。因此，这些结果意味着CLE 14/CLE 20肽可能通过CLV 2-CRN受体激酶起作用，并且它们的可用性和/或丰度可能受到植物中细胞分裂素活性的影响。
Towards an understanding of the interacting nature of the CLAVATA (CLV) complex, we predicted the 3D structures of CLV3/ESR-related (CLE) peptides and the ectodomain of their potential receptor proteins/kinases, and docking models of these molecules. The results show that the ectodomain of CLV1 can form homodimers and that the 12-/13-amino-acid CLV3 peptide fits into the binding clefts of the CLV1 dimers. Our results also demonstrate that the receptor domain of CORYNE (CRN), a recently identified receptor-like kinase, binds tightly to the ectodomain of CLV2, and this likely leads to an increased possibility for docking with CLV1. Furthermore, our docking models reveal that two CRN-CLV2 ectodomain heterodimers are able to form a tetramer receptor complex. Peptides of CLV3, CLE14, CLE19, and CLE20 are also able to bind a potential CLV2-CRN heterodimer or heterotetramer complex. Using a cell-division reporter line, we found that synthetic 12-amino-acid CLE14 and CLE20 peptides inhibit, irreversibly, root growth by reducing cell division rates in the root apical meristem, resulting in a short-root phenotype. Intriguingly, we observed that exogenous application of cytokinin can partially rescue the short-root phenotype induced by over-expression of either CLE14 or CLE20 in planta. However, cytokinin treatment does not rescue the short-root phenotype caused by exogenous application of the synthetic CLE14/CLE20 peptides, suggesting a requirement for a condition provided only in living plants. These results therefore imply that the CLE14/CLE20 peptides may act through the CLV2-CRN receptor kinase, and that their availabilities and/or abundances may be affected by cytokinin activity in planta.
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影响因子：
11.6
作者：
Fiers, M;Golemiec, E;Liu, CM
通讯作者：
Liu, CM
影响因子：
14.9
作者：
Schneidman-Duhovny D;Inbar Y;Nussinov R;Wolfson HJ
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Wolfson HJ
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Fleck C
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Doerner, P
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作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
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Sakagami, Youji