Simultaneous identification of viruses and viral variants with programmable DNA nanobait.
Simultaneous identification of viruses and viral variants with programmable DNA nanobait.
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DOI：
10.1038/s41565-022-01287-x
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发表时间：
2023-03
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
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作者：
呼吸道感染是全世界传染病死亡的主要原因。多重诊断方法是必不可少的，因为许多呼吸道病毒具有难以区分的症状。我们创建了自组装DNA纳米带，可以同时识别多个短RNA靶标。纳米比特方法依赖于通过支点介导的链置换进行的特定靶点选择和通过纳米孔传感进行的快速读出。在这里，我们表明，该平台可以同时识别几种常见的呼吸道病毒，检测来自多种病毒的病毒核酸的一组短靶标。我们的纳米带可以很容易地重新编程，以单核苷酸分辨率区分病毒变体，正如我们对几个关键的SARS-CoV-2变体所证明的那样。最后，我们表明，nanobait区分从阴性和阳性SARS-CoV-2患者的口咽拭子中提取的样品，而无需预扩增。我们的系统允许对天然RNA分子进行多重识别，为在单一检测中诊断多种呼吸道病毒提供了一种新的可扩展方法。临床上用于鉴定呼吸道病毒感染的基于聚合酶链反应的方法不能同时检测多种病毒或病毒变体。在这里，纳米孔核酸传感耦合到可编程核糖核酸酶，以同时识别多种病毒的特定短RNA序列，而无需预检测步骤，如样品纯化或预扩增。
Respiratory infections are the major cause of death from infectious disease worldwide. Multiplexed diagnostic approaches are essential as many respiratory viruses have indistinguishable symptoms. We created self-assembled DNA nanobait that can simultaneously identify multiple short RNA targets. The nanobait approach relies on specific target selection via toehold-mediated strand displacement and rapid readout via nanopore sensing. Here we show that this platform can concurrently identify several common respiratory viruses, detecting a panel of short targets of viral nucleic acids from multiple viruses. Our nanobait can be easily reprogrammed to discriminate viral variants with single-nucleotide resolution, as we demonstrated for several key SARS-CoV-2 variants. Last, we show that the nanobait discriminates between samples extracted from oropharyngeal swabs from negative- and positive-SARS-CoV-2 patients without preamplification. Our system allows for the multiplexed identification of native RNA molecules, providing a new scalable approach for the diagnostics of multiple respiratory viruses in a single assay. Polymerase-chain-reaction-based methods used in clinic to identify respiratory viral infections cannot simultaneously detect multiple viruses or viral variants. Here nanopore nucleic acid sensing is coupled to programmable ribonuclease to simultaneously identify specific short RNA sequences of multiple viruses, without predetection steps such as sample purification or preamplification.
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DOI：
10.1016/j.cej.2021.132966
发表时间：
2022-02-15
期刊：
Chemical engineering journal (Lausanne, Switzerland : 1996)
影响因子：
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作者：
Kumar N;Shetti NP;Jagannath S;Aminabhavi TM
通讯作者：
Aminabhavi TM
影响因子：
28.1
作者：
Arizti-Sanz, Jon;Bradley, A'Doriann;Zhang, Yibin B.;Boehm, Chloe K.;Freije, Catherine A.;Grunberg, Michelle E.;Kosoko-Thoroddsen, Tinna-Solveig F.;Welch, Nicole L.;Pillai, Priya P.;Mantena, Sreekar;Kim, Gaeun;Uwanibe, Jessica N.;John, Oluwagboadurami G.;Eromon, Philomena E.;Kocher, Gregory;Gross, Robin;Lee, Justin S.;Hensley, Lisa E.;MacInnis, Bronwyn L.;Johnson, Jeremy;Springer, Michael;Happi, Christian T.;Sabeti, Pardis C.;Myhrvold, Cameron
通讯作者：
Myhrvold, Cameron
影响因子：
--
作者：
Maglia, Giovanni;Heron, Andrew J.;Stoddart, David;Japrung, Deanpen;Bayley, Hagan
通讯作者：
Bayley, Hagan
用于病毒检测的增强的等温扩增测定法。
影响因子：
16.6
作者：
Qian J;Boswell SA;Chidley C;Lu ZX;Pettit ME;Gaudio BL;Fajnzylber JM;Ingram RT;Ward RH;Li JZ;Springer M
通讯作者：
Springer M
DOI：
10.1038/s41579-021-00535-6
发表时间：
2021-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Leung NHL
通讯作者：
Leung NHL