Focal reversible deactivation of cerebral metabolism affects water diffusion.
Focal reversible deactivation of cerebral metabolism affects water diffusion.
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DOI：
10.1002/mrm.21810
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发表时间：
2008-11
影响因子：
3.3
通讯作者：
Vanduffel, Wim
中科院分区：
文献类型：
--
作者：
Khachaturian, Mark H.;Arsenault, John;Ekstrom, Leeland B.;Tuch, David S.;Vanduffel, Wim
影响大脑扩散对比的潜在生物物理机制仍然知之甚少。我们假设脑代谢可能影响脑弥散对比。本研究旨在建立脑代谢可逆失活的方法，并测量其对MRI弥散信号的影响。我们开发了一种mri兼容的皮层冷却系统，以可逆地使恒河猴V1区皮层代谢失活，并使用磁共振测温法计算大脑的三维温度图，以确定体内大脑失活的程度。表观扩散系数（ADC）的显著变化仅在皮质冷却至低于代谢截止温度20°C的实验中观察到。在温度不变的区域，在皮质冷却期间观察到ADC降低（12-20%）。测量了归一化体内ADC随温度的变化，发现在温度高于20°C时与游离水的归一化ADC相当，但在温度低于20°C时显著降低（降低20 - 25%）。分数各向异性未见变化。在未来的实验中，我们将应用该方法量化可逆性失活对神经活动的影响，通过血流动力学反应来测量，并比较水扩散变化和血流动力学变化。
The underlying biophysical mechanisms which affect cerebral diffusion contrast remain poorly understood. We hypothesized that cerebral metabolism may affect cerebral diffusion contrast. The purpose of this study was to develop the methodology to reversibly deactivate cerebral metabolism and measure the effect on the diffusion MRI signal. We developed an MRI-compatible cortical cooling system to reversibly deactivate cortical metabolism in rhesus monkey area V1 and used MR thermometry to calculate three-dimensional temperature maps of the brain to define the extent of deactivated brain in vivo. Significant changes in the apparent diffusion coefficient (ADC) were only observed during those experiments in which the cortex was cooled below the metabolic cutoff temperature of 20°C. ADC decreases (12–20%) were observed during cortical cooling in regions where the temperature did not change. The normalized in vivo ADC as function of temperature was measured and found to be equivalent to the normalized ADC of free water at temperatures above 20°C, but was significantly decreased below 20°C (20–25% decrease). No changes in fractional anisotropy were observed. In future experiments, we will apply this methodology to quantify the effect of reversible deactivation on neural activity as measured by the hemodynamic response and compare water diffusion changes with hemodynamic changes.
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影响因子：
14.5
作者：
LEENDERS, KL;PERANI, D;JONES, T
通讯作者：
JONES, T
影响因子：
3
作者：
Lomber, SG;Payne, BR;Horel, JA
通讯作者：
Horel, JA
影响因子：
5.7
作者：
Dale, AM;Fischl, B;Sereno, MI
通讯作者：
Sereno, MI
影响因子：
3
作者：
Lomber, SG
通讯作者：
Lomber, SG
DOI：
10.1002/neu.480060404
发表时间：
1975-01-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
BRIMIJOIN, S
通讯作者：
BRIMIJOIN, S