Synteny between Zoysia japonica Steud. and Oryza sativa L. based on RFLP, CISP and PLUG markers
Synteny between Zoysia japonica Steud. and Oryza sativa L. based on RFLP, CISP and PLUG markers
复制标题
结缕草之间的同线性。
DOI：
10.1007/s10722-014-0196-0
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发表时间：
2015-08
影响因子：
2
通讯作者：
Cai, Hongwei
中科院分区：
文献类型：
--
作者：
Wang, Yunwen;Sun, Yan;Mao, Peisheng;Cai, Hongwei
为了研究结缕草与水稻的共线性关系，在前期构建的高密度SSR连锁图谱基础上，增加了限制性片段长度多态性、保守内含子扫描引物和基于PCR的界标独特基因3种标记。将与水稻基因组同源的100个位点整合到我们构建的结缕草SSR连锁图谱中。新图谱比以往报道的所有结缕草遗传图谱都长，连锁群数为20，与结缕草的单倍体染色体数相等。将新定位的100个同源位点定位到除LG20外的19个连锁群上，其中结缕草的第2、8和13个连锁群分别与水稻的第3、6和1染色体共线性，其它连锁群所含标记数不一，与水稻基因组同源。这些结果表明结缕草与水稻之间存在同线关系。本研究构建的遗传连锁图谱和新标记可作为结缕草遗传连锁图谱的参考，为定位与多种性状相关的QTL和改进结缕草育种方案提供依据。
In order to study the synteny between Zoysia japonica and Oryza sativa, we added three types of markers including Restriction fragment length polymorphism, Conserved intron scanning primers and PCR-based landmark unique gene in our previous high-density SSR-based linkage map. A total of 100 loci showed homology to the rice genome were merged into our previously constructed SSR-based linkage map of zoysiagrass. The new map was longer than all zoysiagrass genetic maps reported previously, and contains the number of linkage groups 20, which is equal to the haploid number of chromosomes of zoysiagrass. The new mapped 100 homologous loci were mapped to 19 linkage groups (LGs) except of LG 20, of them, the LGs 2, 8 and 13 of zoysiagrass showed colinearity with chromosomes 3, 6 and 1 of rice respectively, and other LGs of zoysiagrass contained variable numbers of markers that showed homology with the rice genome. These results suggested a syntenic relationship between zoysiagrass and rice. The improved genetic linkage map and the new markers developed in this study will serve as reference genetic linkage maps for zoysiagrass species and will be useful for mapping QTLs associated with various traits, and for improving zoysiagrass breeding programs.
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影响因子：
4.4
作者：
King J;Thomas A;James C;King I;Armstead I
通讯作者：
Armstead I
影响因子：
5.4
作者：
M. Yaneshita;S. Kaneko;T. Sasakuma
通讯作者：
M. Yaneshita;S. Kaneko;T. Sasakuma
DOI：
10.1007/bf02172406
发表时间：
1996-10
期刊：
Molecular and General Genetics MGG
影响因子：
--
作者：
O. Panaud;X. Chen;S. McCouch
通讯作者：
O. Panaud;X. Chen;S. McCouch
影响因子：
1.9
作者：
Li, Manli;Yuyama, Nana;Cai, Hongwei
通讯作者：
Cai, Hongwei
影响因子：
1.3
作者：
Guan, Xuanli;Hirata, Mariko;Cai, Hongwei
通讯作者：
Cai, Hongwei