A High-Dimensional Quantification of Mouse Defensive Behaviors Reveals Enhanced Diversity and Stimulus Specificity.
A High-Dimensional Quantification of Mouse Defensive Behaviors Reveals Enhanced Diversity and Stimulus Specificity.
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DOI：
10.1016/j.cub.2020.09.007
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发表时间：
2020-12-07
期刊：
影响因子：
--
通讯作者：
Lucas RJ
中科院分区：
文献类型：
--
作者：
Storchi R;Milosavljevic N;Allen AE;Zippo AG;Agnihotri A;Cootes TF;Lucas RJ
本能的防御行为，包括刻板的动作和姿势序列，是小鼠行为库的重要组成部分。由于防御行为可以由威胁性的感官刺激可靠地触发，因此最适当的动作的选择取决于刺激的性质。然而，由于小鼠具有广泛的运动动作，因此不清楚哪组运动和姿势代表相关动作。到目前为止，这已经被经验地确定为运动状态的变化。然而，在何种程度上运动单独捕获防御行为的多样性和它们的感官特异性是未知的。为了解决这个问题，我们开发了一种方法来获得小鼠身体的忠实3D重建，从而能够量化各种各样的运动动作。这种更高维度的描述揭示了防御行为比运动数据所指示的更具刺激特异性。因此，对不同刺激的反应在运动方面是等同的（例如，由隐现和声音引起的冻结）可以沿沿着其它维度区分。增强的刺激特异性解释了一个令人惊讶的多样性。聚类分析显示，不同的组合的动作和姿势，引起至少7种不同的行为，需要考虑刺激特异性。此外，每个刺激诱发了不止一种行为，揭示了感觉和行为之间强大的一对多映射，这在运动数据中并不明显。我们的研究结果表明，多样性和感觉特异性的小鼠防御行为展开在一个更高的维度空间，跨越多个运动动作。鲁棒的3D重建被开发用于量化姿势和运动对视觉和听觉威胁的反应包括多个运动动作反应比运动更明显的刺激特异性每个刺激引起不止一个不同的行为反应Storchi et al.将本能防御行为的研究扩展到运动状态的简单量化之外。通过对小鼠姿势和运动进行高维量化，他们发现对视觉和听觉威胁的防御反应比以前设想的更加多样化和刺激特异性。
Instinctive defensive behaviors, consisting of stereotyped sequences of movements and postures, are an essential component of the mouse behavioral repertoire. Since defensive behaviors can be reliably triggered by threatening sensory stimuli, the selection of the most appropriate action depends on the stimulus property. However, since the mouse has a wide repertoire of motor actions, it is not clear which set of movements and postures represent the relevant action. So far, this has been empirically identified as a change in locomotion state. However, the extent to which locomotion alone captures the diversity of defensive behaviors and their sensory specificity is unknown. To tackle this problem, we developed a method to obtain a faithful 3D reconstruction of the mouse body that enabled to quantify a wide variety of motor actions. This higher dimensional description revealed that defensive behaviors are more stimulus specific than indicated by locomotion data. Thus, responses to distinct stimuli that were equivalent in terms of locomotion (e.g., freezing induced by looming and sound) could be discriminated along other dimensions. The enhanced stimulus specificity was explained by a surprising diversity. A clustering analysis revealed that distinct combinations of movements and postures, giving rise to at least 7 different behaviors, were required to account for stimulus specificity. Moreover, each stimulus evoked more than one behavior, revealing a robust one-to-many mapping between sensations and behaviors that was not apparent from locomotion data. Our results indicate that diversity and sensory specificity of mouse defensive behaviors unfold in a higher dimensional space, spanning multiple motor actions. Robust 3D reconstruction is developed to quantify posture and movements Responses to visual and auditory threats encompass multiple motor actions Responses are more stimulus specific than apparent from locomotion Each stimulus evokes more than one distinct behavioral response Storchi et al. extend the study of instinctive defensive behaviors beyond the simple quantification of the locomotor state. By developing a high-dimensional quantification of mouse postures and movements, they show that defensive responses to visual and auditory threats are more diverse and stimulus specific than previously envisaged.
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影响因子：
7.7
作者：
Cande J;Namiki S;Qiu J;Korff W;Card GM;Shaevitz JW;Stern DL;Berman GJ
通讯作者：
Berman GJ
影响因子：
16.6
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Chou XL;Wang X;Zhang ZG;Shen L;Zingg B;Huang J;Zhong W;Mesik L;Zhang LI;Tao HW
通讯作者：
Tao HW
DOI：
10.1073/pnas.1607601113
发表时间：
2016-10-18
影响因子：
11.1
作者：
Berman, Gordon J.;Bialek, William;Shaevitz, Joshua W.
通讯作者：
Shaevitz, Joshua W.
影响因子：
16.2
作者：
Klaus A;Martins GJ;Paixao VB;Zhou P;Paninski L;Costa RM
通讯作者：
Costa RM
DOI：
10.1073/pnas.231499798
发表时间：
2001-11-20
影响因子：
11.1
作者：
Edelman, GM;Gally, JA
通讯作者：
Gally, JA