The immune gene repertoire of an important viral reservoir, the Australian black flying fox.
The immune gene repertoire of an important viral reservoir, the Australian black flying fox.
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DOI：
10.1186/1471-2164-13-261
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发表时间：
2012-06-20
期刊：
影响因子：
4.4
通讯作者：
Wang LF
中科院分区：
文献类型：
--
作者：
Papenfuss AT;Baker ML;Feng ZP;Tachedjian M;Crameri G;Cowled C;Ng J;Janardhana V;Field HE;Wang LF
蝙蝠是一系列新出现和再出现病毒的天然宿主，包括类似sars的冠状病毒、埃博拉病毒、亨尼帕病毒和狂犬病病毒。然而，控制蝙蝠病毒复制的机制尚不清楚，关于蝙蝠抗病毒免疫的任何方面的信息也很少。蝙蝠转录组的大规模平行测序为快速发现基因提供了机会。尽管目前已对一只蝙蝠和一只蝙蝠的基因组进行了低覆盖率测序，但尚未报道任何蝙蝠物种的转录组学数据集。在这项研究中，我们描述了澳大利亚飞狐的免疫转录组，为鉴定包括抗病毒免疫在内的一系列活动的基因提供了重要的资源。为了了解蝙蝠与病毒共存的适应性，我们重新组装了免疫组织的转录组序列和来自P. alecto的受刺激细胞。我们确定了大约18,600个基因参与广泛的活动，其中表达最高的基因涉及细胞生长和维持，酶活性，细胞成分以及代谢和能量途径。3.5%的蝙蝠转录基因与免疫基因对应，共鉴定了约500个免疫基因，为先天免疫和适应性免疫提供了概述。一小部分转录本与任何公共数据库中的注释序列不匹配，可能代表蝙蝠特异性转录本。这项研究代表了第一个报道的蝙蝠转录组数据集，并提供了对蝙蝠表达基因的调查，补充了现有的蝙蝠基因组数据。此外，这些数据提供了对蝙蝠抗病毒反应相关基因的深入了解，并为研究这些分子在病毒感染免疫反应中的作用奠定了基础。
Bats are the natural reservoir host for a range of emerging and re-emerging viruses, including SARS-like coronaviruses, Ebola viruses, henipaviruses and Rabies viruses. However, the mechanisms responsible for the control of viral replication in bats are not understood and there is little information available on any aspect of antiviral immunity in bats. Massively parallel sequencing of the bat transcriptome provides the opportunity for rapid gene discovery. Although the genomes of one megabat and one microbat have now been sequenced to low coverage, no transcriptomic datasets have been reported from any bat species. In this study, we describe the immune transcriptome of the Australian flying fox, Pteropus alecto, providing an important resource for identification of genes involved in a range of activities including antiviral immunity. Towards understanding the adaptations that have allowed bats to coexist with viruses, we have de novo assembled transcriptome sequence from immune tissues and stimulated cells from P. alecto. We identified about 18,600 genes involved in a broad range of activities with the most highly expressed genes involved in cell growth and maintenance, enzyme activity, cellular components and metabolism and energy pathways. 3.5% of the bat transcribed genes corresponded to immune genes and a total of about 500 immune genes were identified, providing an overview of both innate and adaptive immunity. A small proportion of transcripts found no match with annotated sequences in any of the public databases and may represent bat-specific transcripts. This study represents the first reported bat transcriptome dataset and provides a survey of expressed bat genes that complement existing bat genomic data. In addition, these data provide insight into genes relevant to the antiviral responses of bats, and form a basis for examining the roles of these molecules in immune response to viral infection.
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影响因子：
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