The inflation response of the human lamina cribrosa and sclera: Analysis of deformation and interaction.
The inflation response of the human lamina cribrosa and sclera: Analysis of deformation and interaction.
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DOI：
10.1016/j.actbio.2020.01.049
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发表时间：
2020-04-01
影响因子：
9.7
通讯作者：
Nguyen TD
中科院分区：
文献类型：
--
作者：
Midgett DE;Jefferys JL;Quigley HA;Nguyen TD
本研究探讨了无青光眼病史的人死后筛板（LC）和邻近的视乳头周围巩膜（PPS）的膨胀反应。对来自7名供体的13只人眼的后巩膜进行5-45 mmHg的受控加压。使用激光扫描显微镜（LSM）对每个压力下LC和PPS体积内胶原的二次谐波产生（SHG）响应和弹性蛋白的双光子荧光（TPF）响应进行成像。使用数字体积相关（DVC）分析图像体积，以计算压力之间的三维（3D）变形场。与PPS相比，LC在5-45 mmHg之间表现出更大的径向应变Err和最大主应变Emax（p < 0.0001）以及更大的后向位移（p = 0.0007），但具有相似的平均周向应变Eθθ和最大剪切应变Γmax。两种组织的Emax和Γmax在LC-PPS界面附近最高，在鼻象限最低。较大的LC面积与外周LC中较小的Emax和中心LC中较大的Emax相关（p ≤ 0.01）。内部PPS中的Emax、Γmax和Eθθ随着相邻LC区域中应变的增加而增加（p ≤ 0.001）。PPS中较小的应变与PPS和中央LC之间较大的后位移差异相关（Emax和Err p < 0.0001），表明PPS的压力-应变反应较硬与LC的较大后弯曲相关。
This study investigated the inflation response of the lamina cribrosa (LC) and adjacent peripapillary sclera (PPS) in post-mortem human eyes with no history of glaucoma. The posterior sclera of 13 human eyes from 7 donors was subjected to controlled pressurization between 5-45 mmHg. A laser-scanning microscope (LSM) was used to image the second harmonic generation (SHG) response of collagen and the two-photon fluorescent (TPF) response of elastin within the volume of the LC and PPS at each pressure. Image volumes were analyzed using digital volume correlation (DVC) to calculate the three-dimensional (3D) deformation field between pressures. The LC exhibited larger radial strain, Err, and maximum principal strain, Emax, (p < 0.0001) and greater posterior displacement (p = 0.0007) compared to the PPS between 5-45 mmHg, but had similar average circumferential strain, Eθθ, and maximum shear strain, Γmax. The Emax and Γmax were highest near the LC-PPS interface and lowest in the nasal quadrant of both tissues. Larger LC area was associated with smaller Emax in the peripheral LC and larger Emax in the central LC (p ≤ 0.01). The Emax, Γmax, and Eθθ in the inner PPS increased with increasing strain in adjacent LC regions (p ≤ 0.001). Smaller strains in the PPS were associated with a larger difference in the posterior displacement between the PPS and central LC (p < 0.0001 for Emax and Err), indicating that a stiffer pressure-strain response of the PPS is associated with greater posterior bowing of the LC.
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