Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help.
Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help.
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鼠类上皮内淋巴细胞的免疫调节功能：γ/Delta T细胞受体阳性（TCR+）T细胞消除口服耐受性，而α/βTCR+ TCR+ TCR细胞可提供B细胞帮助。
DOI：
10.1084/jem.175.3.695
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发表时间：
1992-03-01
影响因子：
15.3
通讯作者：
KIYONO, H
中科院分区：
文献类型：
--
作者：
FUJIHASHI, K;TAGUCHI, T;AICHER, WK;MCGHEE, JR;BLUESTONE, JA;ELDRIDGE, JH;KIYONO, H
过去的研究表明，具有独特特征的效应 T 细胞子集可以消除半抗原或抗原诱导的耐受性，这种免疫反应的重建被称为反抑制。我们研究了口服耐受 (OT) 模型中的对比抑制，其中过继转移的抗原特异性 T 对比抑制 (Tcs) 细胞逆转 OT 并导致对引发抗原的抗体反应。在本研究中，我们发现，口服绵羊红细胞 (SRBC) 免疫小鼠的鼠上皮内淋巴细胞 (IEL) 含有具有 Tcs 细胞活性的 T 细胞。这种效应是由 CD3+ γ/δ T 细胞受体阳性 (TCR+) 介导的，但不是 α/β TCR+ T 细胞介导的，并且 γ/δ TCR+ Tcs 细胞与 CD4-、CD8+ 和 CD4-、CD8-（双阴性）IEL 分数相关。 CD4-、CD8+ γ/δ TCR+ IEL 进一步分为 Vicia villosa 粘附级分和非粘附级分。将 V. villosa 粘附的 γ/δ TCR+ T 细胞过继转移至 OT 至 SRBC 的小鼠中，导致脾脏 IgA、IgM 和 IgG 亚类抗 SRBC 反应，而 V. villosa 非粘附的 γ/δ TCR+ T 细胞没有活性。 γ/δ TCR+ IEL 不支持 B 细胞培养物中的体外抗体应答，而 α/β TCR+ IEL 是有效的 T 辅助细胞。此外，检查了γ/δ TCR+ IEL产生的细胞因子，发现与α/β TCR+ IEL和V. villosa非粘附性γ/δ TCR+ IEL相比，具有反抑制功能的γ/δ TCR+ V. villosa粘附级分含有低水平的IL-5 mRNA和少量产生IL-5的细胞。我们的结果现在表明，小鼠 IEL 含有两种不同类型的 T 细胞，它们在免疫反应中发挥作用，例如，产生 IL-5 并充当辅助细胞的 α/β TCR+ T 细胞，以及恢复口服抗原耐受小鼠的抗体反应的 γ/δ TCR+ T 细胞。
Past work has shown that a subset of effector T cells with unique characteristics could abrogate hapten- or antigen-induced tolerance, and the reconstitution of this immune response has been termed contrasuppression. We have studied contrasuppression in a model of oral tolerance (OT) in which adoptively transferred antigen-specific T contrasuppressor (Tcs) cells reverse OT and result in antibody responses to the eliciting antigen. In the present study, we show that murine intraepithelial lymphocytes (IELs) from mice orally immunized with sheep red blood cells (SRBC) contain T cells that exhibit Tcs cell activity. This effect was mediated by CD3+ gamma/delta T cell receptor- positive (TCR+), but not alpha/beta TCR+ T cells, and gamma/delta TCR+ Tcs cells were associated with both the CD4-,CD8+ and CD4-,CD8- (double- negative) IEL fractions. The CD4-,CD8+ gamma/delta TCR+ IELs were further separated into Vicia villosa-adherent and -nonadherent fractions. Adoptive transfer of V. villosa-adherent gamma/delta TCR+ T cells to mice with OT to SRBC resulted in splenic IgA, IgM, and IgG subclass anti-SRBC responses, while V. villosa-nonadherent gamma/delta TCR+ T cells were without activity. The gamma/delta TCR+ IELs did not support in vitro antibody responses in B cell cultures, while alpha/beta TCR+ IELs were effective T helper cells. Further, cytokine production by the gamma/delta TCR+ IELs was examined, and the gamma/delta TCR+ V. villosa-adherent fraction, which possessed contrasuppressor function, contained low levels of IL-5 mRNA and small numbers of IL-5-producing cells when compared with alpha/beta TCR+ IELs and V. villosa-nonadherent gamma/delta TCR+ IELs. Our results now show that mouse IELs contain two distinct types of T cells that function in the immune response, e.g., alpha/beta TCR+ T cells that produce IL-5 and function as helper cells, and gamma/delta TCR+ T cells that restore antibody responses in mice that had been orally tolerized with antigen.
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DOI：
10.1084/jem.173.2.471
发表时间：
1991-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guy-Grand D;Cerf-Bensussan N;Malissen B;Malassis-Seris M;Briottet C;Vassalli P
通讯作者：
Vassalli P
DOI：
10.1084/jem.169.6.2133
发表时间：
1989-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Beagley KW;Eldridge JH;Lee F;Kiyono H;Everson MP;Koopman WJ;Hirano T;Kishimoto T;McGhee JR
通讯作者：
McGhee JR
影响因子：
--
作者：
LANIER, LL;GUTMAN, GA;WARNER, NL
通讯作者：
WARNER, NL
影响因子：
64.8
作者：
ASARNOW, DM;GOODMAN, T;ALLISON, JP
通讯作者：
ALLISON, JP
影响因子：
64.8
作者：
BONNEVILLE, M;JANEWAY, CA;TONEGAWA, S
通讯作者：
TONEGAWA, S