Marker-Assisted Development and Evaluation of Near-Isogenic Lines for Broad-Spectrum Powdery Mildew Resistance Gene Pm2b Introgressed into Different Genetic Backgrounds of Wheat.
Marker-Assisted Development and Evaluation of Near-Isogenic Lines for Broad-Spectrum Powdery Mildew Resistance Gene Pm2b Introgressed into Different Genetic Backgrounds of Wheat.
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小麦不同遗传背景广谱白粉病抗性基因 Pm2b 近等基因系的标记辅助开发和评价
DOI：
10.3389/fpls.2017.01322
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发表时间：
2017
影响因子：
5.6
通讯作者：
An D
中科院分区：
文献类型：
--
作者：
Xu H;Cao Y;Xu Y;Ma P;Ma F;Song L;Li L;An D
目前，中国大部分已发布的小麦品种或选育品系对小麦白粉病（Blumeria graminis f. sp. tritici, Bgt）易感，迫切需要将有效的广谱抗白粉病基因快速转移到优良品种/品系中。具有短靶基因区域的近等基因系在分子育种和图谱克隆中具有重要意义，可通过标记辅助选择和常规表型鉴定相结合的方法进行开发。然而，在以往的研究中，没有使用该方法报道Pm基因NILs。从中国小麦选育品系KM2939中分离出一种新的广谱抗性优势基因Pm2b，该基因在小麦苗期和成虫期均具有较高的抗性。本研究利用分子标记的正向和背景选择（FS和BS），通过回交将Pm2b基因渗入到3个优质易感商品品种世麦15、世新828和科农199中。选择合适的回交代，选择适宜的群体规模和标记数，在3个受体基因组组成最高的遗传背景下，通过简单序列重复标记和660K单核苷酸多态性阵列证实Pm2b基因的纯合抗性BC3F2:3 NILs，并对其抗性和农艺性状进行了评价。Pm2b NILs与其相应的复发亲本之间的不同抗性和相似或改善的农艺性能表明它们在Pm2b基因的标记辅助育种中具有潜在的价值。此外，Pm2基因的4个侧翼诊断标记（CFD81、BWM25、BWM20和BWM21）的开发可以有效地辅助Pm2基因的正向选择，加速该抗性基因的转移和利用。
At present, most of released wheat cultivars or breeding lines in China are susceptible to powdery mildew (Pm) (caused by Blumeria graminis f. sp. tritici, Bgt), so there is an urgent need to rapidly transfer effective and broad-spectrum Pm resistance genes into elite cultivars/lines. Near-isogenic lines (NILs) with short target gene region are very important in molecular breeding and map-based cloning and can be developed by combining marker-assisted selection and conventional phenotypic identification. However, no Pm gene NILs were reported by using this method in the previous studies. A new broad-spectrum dominant resistance gene Pm2b, derived from the Chinese wheat breeding line KM2939, conferred high resistance to Pm at both the seedling and adult stages. In this study, with the aid of forward and background selection (FS and BS) using molecular markers, the Pm2b gene was introgressed into three elite susceptible commercial cultivars Shimai 15, Shixin 828, and Kenong 199 through the back-crossing procedure. With the appropriate backcrossing generations, selected population sizes and marker number for BS, the homozygous resistant BC3F2:3 NILs of Pm2b gene in the three genetic backgrounds with the highest recipient genome composition of about 99%, confirmed by simple sequence repeat markers and 660K single nucleotide polymorphic array, were developed and evaluated for the powdery mildew resistance and agronomic traits. The different resistance and similar or improved agronomic performance between Pm2b NILs and their corresponding recurrent parents indicated their potential value in the marker-assisted breeding of the Pm2b gene. Moreover, the development of four flanked diagnostic markers (CFD81, BWM25, BWM20, and BWM21) of the Pm2 gene can effectively assist the forward selection and accelerate the transfer and use of this resistance gene.
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