Antiviral RNA interference in disease vector (Asian longhorned) ticks.
Antiviral RNA interference in disease vector (Asian longhorned) ticks.
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疾病媒介（亚洲长角）蜱中的抗病毒 RNA 干扰
DOI：
10.1371/journal.ppat.1010119
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发表时间：
2021-12
期刊：
影响因子：
6.7
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Xu Y;Zhong Z;Ren Y;Ma L;Ye Z;Gao C;Wang J;Li Y
蚊子和扁虱等病媒在人类和动物病毒病原体的出现和重新出现方面发挥了重要作用。然而，与蚊子相比，人们对扁虱的抗病毒反应知之甚少。在这里，我们发现亚洲长角硬蜱主要产生22核苷酸病毒衍生的siRNAs(VsiRNAs)，以应对通过血液喂养获得的血小板减少综合征病毒(SFTSV)、新村病毒(NOV)或辛德比斯病毒(SINV)的严重发热。值得注意的是，通过胸腔注射获得NOV和SINV的实验也启动了病毒复制，并触发了长角毛虫vsiRNAs的产生。我们证明了缺乏表达其RNAi病毒抑制因子(VSR)的突变体nov在长角虫中的复制水平显著低于野生型nov，但在系统发育比较中发现的tick Dicer2样蛋白被敲除后积累到更高的水平。此外，一组已知的动物VSR在SINV基因组中的顺式表达极大地促进了重组病毒的积累。这项研究建立了一种新的病毒-载体-小鼠实验模型，并提供了第一个体内证据，证明了扁虱的RNAi反应具有抗病毒功能。有趣的是，比较表达绿色荧光蛋白或SFTSV蛋白的SINV重组体的积累水平，确定该病毒非结构蛋白为假定的VSR。了解硬虱体内抗病毒RNAi途径的功能对于了解病毒与宿主的相互作用和控制硬虱传播的病毒病原体至关重要。
Disease vectors such as mosquitoes and ticks play a major role in the emergence and re-emergence of human and animal viral pathogens. Compared to mosquitoes, however, much less is known about the antiviral responses of ticks. Here we showed that Asian longhorned ticks (Haemaphysalis longicornis) produced predominantly 22-nucleotide virus-derived siRNAs (vsiRNAs) in response to severe fever with thrombocytopenia syndrome virus (SFTSV, an emerging tick-borne virus), Nodamura virus (NoV), or Sindbis virus (SINV) acquired by blood feeding. Notably, experimental acquisition of NoV and SINV by intrathoracic injection also initiated viral replication and triggered the production of vsiRNAs in H. longicornis. We demonstrated that a mutant NoV deficient in expressing its viral suppressor of RNAi (VSR) replicated to significantly lower levels than wildtype NoV in H. longicornis, but accumulated to higher levels after knockdown of the tick Dicer2-like protein identified by phylogeny comparison. Moreover, the expression of a panel of known animal VSRs in cis from the genome of SINV drastically enhanced the accumulation of the recombinant viruses. This study establishes a novel model for virus-vector-mouse experiments with longhorned ticks and provides the first in vivo evidence for an antiviral function of the RNAi response in ticks. Interestingly, comparing the accumulation levels of SINV recombinants expressing green fluorescent protein or SFTSV proteins identified the viral non-structural protein as a putative VSR. Elucidating the function of ticks’ antiviral RNAi pathway in vivo is critical to understand the virus-host interaction and the control of tick-borne viral pathogens.
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影响因子：
3.8
作者：
Chen, Xiao-Ping;Cong, Mei-Li;Zhang, Yong-Zhen
通讯作者：
Zhang, Yong-Zhen
影响因子：
5.3
作者：
Grubaugh ND;Rückert C;Armstrong PM;Bransfield A;Anderson JF;Ebel GD;Brackney DE
通讯作者：
Brackney DE
影响因子：
30.5
作者：
Goic, Bertsy;Vodovar, Nicolas;Saleh, Maria-Carla
通讯作者：
Saleh, Maria-Carla
影响因子：
16.6
作者：
Gulia-Nuss M;Nuss AB;Meyer JM;Sonenshine DE;Roe RM;Waterhouse RM;Sattelle DB;de la Fuente J;Ribeiro JM;Megy K;Thimmapuram J;Miller JR;Walenz BP;Koren S;Hostetler JB;Thiagarajan M;Joardar VS;Hannick LI;Bidwell S;Hammond MP;Young S;Zeng Q;Abrudan JL;Almeida FC;Ayllón N;Bhide K;Bissinger BW;Bonzon-Kulichenko E;Buckingham SD;Caffrey DR;Caimano MJ;Croset V;Driscoll T;Gilbert D;Gillespie JJ;Giraldo-Calderón GI;Grabowski JM;Jiang D;Khalil SMS;Kim D;Kocan KM;Koči J;Kuhn RJ;Kurtti TJ;Lees K;Lang EG;Kennedy RC;Kwon H;Perera R;Qi Y;Radolf JD;Sakamoto JM;Sánchez-Gracia A;Severo MS;Silverman N;Šimo L;Tojo M;Tornador C;Van Zee JP;Vázquez J;Vieira FG;Villar M;Wespiser AR;Yang Y;Zhu J;Arensburger P;Pietrantonio PV;Barker SC;Shao R;Zdobnov EM;Hauser F;Grimmelikhuijzen CJP;Park Y;Rozas J;Benton R;Pedra JHF;Nelson DR;Unger MF;Tubio JMC;Tu Z;Robertson HM;Shumway M;Sutton G;Wortman JR;Lawson D;Wikel SK;Nene VM;Fraser CM;Collins FH;Birren B;Nelson KE;Caler E;Hill CA
通讯作者：
Hill CA
影响因子：
4.2
作者：
Campbell CL;Keene KM;Brackney DE;Olson KE;Blair CD;Wilusz J;Foy BD
通讯作者：
Foy BD