Use of the light/dark test for anxiety in adult and adolescent male rats.
Use of the light/dark test for anxiety in adult and adolescent male rats.
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DOI：
10.1016/j.bbr.2013.05.035
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发表时间：
2013-11-01
影响因子：
2.7
通讯作者：
Kuhn, Cynthia M.
中科院分区：
文献类型：
--
作者：
Arrant, Andrew E.;Schramm-Sapyta, Nicole L.;Kuhn, Cynthia M.
光/暗（LD）测试是一种常用的啮齿动物无条件焦虑样行为测试，其基于探索新区域的驱动力与对明亮明亮开放空间的厌恶之间的接近/回避冲突。我们使用LD测试来研究青少年（出生后第28-34天（PN））和成年（PN 67 -74）雄性大鼠之间行为的发育差异。我们调查是否LD行为措施反映焦虑样行为在每个年龄组相似，使用因素分析和多元回归。这些分析表明，在光室中的时间，在光中的百分比距离，饲养，和潜伏期出现到光室的焦虑样行为的措施，在每个年龄组，而总的距离和在黑暗的车厢距离提供的运动活动的指数。然后，我们使用这些措施来评估基线LD行为和抗焦虑药物的反应的发展差异。青春期的大鼠比成年大鼠更快地进入光室，并且更少地戳入光室。这些年龄差异可能反映了青少年大鼠比成年大鼠更大的风险承担和更少的风险评估。与成年大鼠相比，青少年大鼠对苯二氮卓类反向激动剂N-甲基-β-咔啉-3-甲酰胺（FG-7142）和α2肾上腺素能拮抗剂育亨宾对焦虑样行为的致焦虑作用的敏感性较低，但运动变量受到类似的影响。这些数据支持因子分析的结果，并表明，GABA能和去甲肾上腺素能调制LD焦虑样行为可能是不成熟的青春期。
The light/dark (LD) test is a commonly used rodent test of unconditioned anxiety-like behavior that is based on an approach/avoidance conflict between the drive to explore novel areas and an aversion to brightly lit, open spaces. We used the LD test to investigate developmental differences in behavior between adolescent (postnatal day (PN) 28–34) and adult (PN67–74) male rats. We investigated whether LD behavioral measures reflect anxiety-like behavior similarly in each age group using factor analysis and multiple regression. These analyses showed that time in the light compartment, percent distance in the light, rearing, and latency to emerge into the light compartment were measures of anxiety-like behavior in each age group, while total distance traveled and distance in the dark compartment provided indices of locomotor activity. We then used these measures to assess developmental differences in baseline LD behavior and the response to anxiogenic drugs. Adolescent rats emerged into the light compartment more quickly than adults and made fewer pokes into the light compartment. These age differences could reflect greater risk taking and less risk assessment in adolescent rats than adults. Adolescent rats were less sensitive than adults to the anxiogenic effects of the benzodiazepine inverse agonist N-methyl-β-carboline-3-carboxamide (FG-7142) and the α2 adrenergic antagonist yohimbine on anxiety-like behaviors validated by factor analysis, but locomotor variables were similarly affected. These data support the results of the factor analysis and indicate that GABAergic and noradrenergic modulation of LD anxiety-like behavior may be immature during adolescence.
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影响因子：
2.9
作者：
Andrade, MMM;Tomé, MF;de Andrade, TGCS
通讯作者：
de Andrade, TGCS
影响因子：
3.4
作者：
Caster, JM;Walker, QD;Kuhn, CM
通讯作者：
Kuhn, CM
影响因子：
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作者：
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通讯作者：
Spear, LP
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作者：
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通讯作者：
COMMISSARIS, RL
影响因子：
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作者：
File, Sandra E;Lippa, Arnold S;Lippa, Morgen T
通讯作者：
Lippa, Morgen T