Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination.
Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination.
复制标题
DOI：
10.1016/j.vetimm.2006.10.005
复制
发表时间：
2007-01-15
影响因子：
1.8
通讯作者：
Tsujimoto H
中科院分区：
文献类型：
--
作者：
Ohmori K;Masuda K;DeBoer DJ;Sakaguchi M;Tsujimoto H
我们先前证明了在接种疫苗后发生过敏反应的犬血清中存在针对胎牛血清（FCS）的IgE。在这项研究中，通过免疫印迹分析，我们调查了免疫球蛋白E反应成分的FCS使用血清从16只狗表现出过敏反应后接种。免疫印迹分析显示，约25-、50-、66-、75-、120-和175-kDa的几种FCS蛋白与显示接种后过敏反应的犬血清中的IgE强烈反应。在FCS免疫印迹分析中分析的16份犬血清样品中，在14份的血清中检测到66-kDa条带，根据其分子量推测其为白蛋白;然而，在14份犬样品中仅在4份中检测到血清IgE与牛血清白蛋白的反应性。这些结果表明，包括白蛋白在内的多种FCS组分可作为犬的过敏原，在接种后发生过敏反应。
We previously demonstrated the presence of IgE directed to fetal calf serum (FCS) in the sera from dogs that developed allergic reactions after vaccination. In this study, by an immunoblot analysis, we investigated the IgE-reactive components of FCS using sera from 16 dogs that exhibited allergic reactions after vaccination. The immunoblot analysis revealed that several FCS proteins of approximately 25-, 50-, 66-, 75-, 120-, and 175-kDa strongly reacted with IgE in the sera from dogs that showed post-vaccination allergic reactions. The 66-kDa band was detected in the sera from 14 of the 16 dog serum samples analyzed in the immunoblot analysis for FCS, and it was speculated to be albumin based on its molecular weight; however, serum IgE reactivity to bovine serum albumin could be detected in only four of the 14 dog samples. These findings demonstrated that a variety of FCS components including albumin could function as allergens in dogs that developed allergic reactions after vaccination.
登录
查看更多内容
影响因子：
32.4
作者：
Mukai, K;Matsuoka, K;Karasuyama, H
通讯作者：
Karasuyama, H
影响因子：
1
作者：
HogenEsch, H;Dunham, AD;DeBoer, DJ
通讯作者：
DeBoer, DJ
影响因子：
2.2
作者：
Ohmori, K;Sakaguchi, M;Tsujimoto, H
通讯作者：
Tsujimoto, H
影响因子：
5.5
作者：
Ozaki, T;Nishimura, N;Akiyama, M
通讯作者：
Akiyama, M
影响因子：
1.8
作者：
DEBOER, DJ;EWING, KM;SCHULTZ, KT
通讯作者：
SCHULTZ, KT