The conserved global regulator VeA is necessary for symptom production and mycotoxin synthesis in maize seedlings by Fusarium verticillioides.
The conserved global regulator VeA is necessary for symptom production and mycotoxin synthesis in maize seedlings by Fusarium verticillioides.
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DOI：
10.1111/j.1365-3059.2011.02504.x
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发表时间：
2012-02-01
期刊：
影响因子：
2.7
通讯作者：
Calvo AM
中科院分区：
文献类型：
--
作者：
Myung K;Zitomer NC;Duvall M;Glenn AE;Riley RT;Calvo AM
veA或天鹅绒基因是曲霉属物种中真菌毒素和其他次级代谢产物的生物合成所必需的。此外，veA也已被证明是必需的正常种子定殖在黄曲霉和寄生曲霉。目前的研究表明，veA同源物广泛分布在真菌，特别是在子囊菌。轮枝镰孢veA直系同源物FvVE 1也是合成多种次级代谢产物（包括伏马菌素和镰孢菌素）所必需的。该研究还表明，从接种FvVE 1缺失突变体的种子生长的玉米植株没有表现出疾病症状，而从接种F.轮枝菌属野生型和互补菌株在相同的实验条件下清楚地显示出疾病症状。在后一种情况下，病变的存在与伏马菌素在植物组织中的积累相一致，并且只有这些植物组织具有升高的鞘氨醇碱及其1-磷酸衍生物水平，表明神经酰胺合酶的抑制和鞘脂代谢的破坏。结果表明，FvVE 1是F.轮枝霉对玉米幼苗的危害。veA同源物在子囊菌中的保守性表明veA在调节其他真菌的次生代谢和相关致病性中可能发挥关键作用。
The veA or velvet gene is necessary for biosynthesis of mycotoxins and other secondary metabolites in Aspergillus species. In addition, veA has also been demonstrated to be necessary for normal seed colonization in Aspergillus flavus and Aspergillus parasiticus. The present study shows that veA homologues are broadly distributed in fungi, particularly in Ascomycetes. The Fusarium verticillioides veA orthologue, FvVE1, is also required for the synthesis of several secondary metabolites, including fumonisin and fusarins. This study also shows that maize plants grown from seeds inoculated with FvVE1 deletion mutants did not show disease symptoms, while plants grown from seeds inoculated with the F. verticillioides wildtype and complementation strains clearly showed disease symptoms under the same experimental conditions. In this latter case, the presence of lesions coincided with accumulation of fumonisins in the plant tissues, and only these plant tissues had elevated levels of sphingoid bases and their 1-phosphate derivatives, indicating inhibition of ceramide synthase and disruption of sphingolipid metabolism. The results strongly suggest that FvVE1 is necessary for pathogenicity by F. verticillioides against maize seedlings. The conservation of veA homologues among ascomycetes suggests that veA could play a pivotal role in regulating secondary metabolism and associated pathogenicity in other fungi.
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影响因子：
3.6
作者：
Wiemann P;Brown DW;Kleigrewe K;Bok JW;Keller NP;Humpf HU;Tudzynski B
通讯作者：
Tudzynski B
影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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