Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress.
Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress.
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DOI：
10.3390/ijms19041216
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发表时间：
2018-04-17
影响因子：
5.6
通讯作者：
Zhuo RY
中科院分区：
文献类型：
--
作者：
Chen SS;Jiang J;Han XJ;Zhang YX;Zhuo RY
东南景天是一种镉、锌、铅共积累的植物，能积累大量的重金属离子，没有明显的毒害迹象，是一种很有前途的植物修复植物。热休克转录因子(HSF)家族在植物的生长发育和逆境反应中发挥着重要作用。虽然一些Hsf在非生物胁迫中的作用已经在模式植物中得到了很好的研究，但在重金属超积累植物中Hsf家族还没有得到系统的研究。在此，我们基于Cd胁迫下的转录组对阿尔弗雷迪链霉菌的HSF基因家族进行了全面的分析。有22个HSF成员被鉴定，并在系统发育上分为3个类别，即SaHsfA、SaHsfB和SaHsfC。这三个班级都有相似的主题。22个HSF成员的表达谱有显著差异：18个SaHsf对Cd胁迫有反应，多个SaHsp基因，包括SaHsp18.1、SaHsp22、SaHsp26.5、SaHsp70、SaHsp90和SaHsp101。两个A4类成员SaHsfA4a和SaHsfA4c具有转录激活活性。SaHsfA4a和SaHsfA4c在转基因酵母中的过表达表明，转基因酵母对Cd胁迫和Cd积累的耐受性提高。我们的结果表明，SaHsf在重金属胁迫反应中起着重要的调节作用，为进一步研究SaHsf对重金属胁迫的调控机制提供了参考。
Sedum alfredii Hance, a cadmium (Cd)/zinc (Zn)/lead (Pb) co-hyperaccumulating species, is a promising phytoremediation candidate because it accumulates substantial amounts of heavy metal ions without showing any obvious signs of poisoning. The heat shock transcription factor (Hsf) family plays crucial roles in plant growth, development, and stress responses. Although the roles of some Hsfs in abiotic stress have been well studied in model plants, the Hsf family has not been systematically investigated in heavy metal hyperaccumulators. Here, we comprehensively analyzed the Hsf gene family in S. alfredii based on a transcriptome under Cd stress. There were 22 Hsf members that were identified and phylogenetically clustered into three classes, namely, SaHsfA, SaHsfB, and SaHsfC. All of the three classes shared similar motifs. The expression profiles of the 22 Hsf members showed significant differences: 18 SaHsfs were responsive to Cd stress, as were multiple SaHsp genes, including SaHsp18.1, SaHsp22, SaHsp26.5, SaHsp70, SaHsp90, and SaHsp101. Two class A4 members, SaHsfA4a and SaHsfA4c, exhibited transcriptional activation activities. Overexpression of SaHsfA4a and SaHsfA4c in transgenic yeast indicated an improved tolerance to Cd stress and Cd accumulation. Our results suggest SaHsfs play important regulatory roles in heavy metal stress responses, and provide a reference for further studies on the mechanism of heavy metal stress regulation by SaHsfs.
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影响因子：
5.3
作者：
Personat JM;Tejedor-Cano J;Prieto-Dapena P;Almoguera C;Jordano J
通讯作者：
Jordano J
影响因子：
5.6
作者：
Cao F;Cheng H;Cheng S;Li L;Xu F;Yu W;Yuan H
通讯作者：
Yuan H
IBS：用于生物序列呈现和可视化的插画家
DOI：
10.1093/bioinformatics/btv362
发表时间：
2015-10-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Liu W;Xie Y;Ma J;Luo X;Nie P;Zuo Z;Lahrmann U;Zhao Q;Zheng Y;Zhao Y;Xue Y;Ren J
通讯作者：
Ren J
影响因子：
5.6
作者：
Li Z;Han X;Song X;Zhang Y;Jiang J;Han Q;Liu M;Qiao G;Zhuo R
通讯作者：
Zhuo R
玉米热休克转录因子基因 ZmHsf04 的异位过度表达赋予转基因拟南芥增加的热和盐胁迫耐受性
影响因子：
2.6
作者：
Jiang, Yingli;Zheng, Qianqian;Wu, Jiandong
通讯作者：
Wu, Jiandong