Survey of the infant male urobiome and genomic analysis of Actinotignum spp.
Survey of the infant male urobiome and genomic analysis of Actinotignum spp.
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DOI：
10.1038/s41522-023-00457-6
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发表时间：
2023-12-01
影响因子：
9.2
通讯作者：
Hadjifrangiskou, Maria
中科院分区：
文献类型：
--
作者：
Reasoner, Seth A.;Flores, Viktor;Van Horn, Gerald;Morales, Grace;Peard, Leslie M.;Abelson, Benjamin;Manuel, Carmila;Lee, Jessica;Baker, Bailey;Williams, Timothy;Schmitz, Jonathan E.;Clayton, Douglass B.;Hadjifrangiskou, Maria
膀胱内有一个被称为尿生物群系的微生物群落，目前还没有得到充分的研究。在这项研究中，我们提出了健康的男性婴儿的尿生物群系从经尿道导管插入术收集的样本。使用增强培养和扩增子测序的组合，我们确定了几种常见的细菌属，可以进一步研究它们对整个生命周期的泌尿健康的影响。所有样本之间共享许多属，表明该队列中的尿生物群组成一致。我们注意到，对于这个队列，生命早期暴露，包括出生方式（阴道与剖腹产）或既往抗生素暴露，并不影响尿生物群系的组成。此外，我们报告了从这些雄性婴儿膀胱中分离出的可培养细菌，包括放线菌属，一种与老年男性尿路感染有关的细菌属。在此，我们分离并测序了9个不同的放线菌菌株。增强了围绕该属的基因组知识，并开辟了描绘该尿生物组成分的微生物学的途径。此外，我们提出了一个框架，使用文化依赖性和测序方法的组合，在urobiome的发现机制。
The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Cesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including Actinotignum spp., a bacterial genus that has been associated with urinary tract infections in older male adults. Herein, we isolate and sequence 9 distinct strains of Actinotignum spp. enhancing the genomic knowledge surrounding this genus and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.6
作者：
Ammitzbøll N;Bau BPJ;Bundgaard-Nielsen C;Villadsen AB;Jensen AM;Leutscher PDC;Glavind K;Hagstrøm S;Arenholt LTS;Sørensen S
通讯作者：
Sørensen S
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
DOI：
10.1016/j.ejogrb.2017.04.040
发表时间：
2017-07-01
影响因子：
2.6
作者：
Curtiss, Natasha;Balachandran, Aswini;Duckett, Jonathan
通讯作者：
Duckett, Jonathan
影响因子：
9.4
作者：
Hilt, Evann E.;McKinley, Kathleen;Schreckenberger, Paul C.
通讯作者：
Schreckenberger, Paul C.