The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection.
The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection.
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DOI：
10.1371/journal.pone.0131615
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zeichner SL
中科院分区：
文献类型：
--
作者：
Goldberg BE;Mongodin EF;Jones CE;Chung M;Fraser CM;Tate A;Zeichner SL
口腔微生物群落（微生物群）在人类健康和疾病中起着至关重要的作用。口腔微生物群的改变可能与诸如牙龈炎、牙周炎、儿童龋齿、牙槽骨炎、口腔念珠菌病和牙髓感染的病症相关。在免疫抑制人群中，潜在口腔疾病的范围甚至更广，包括念珠菌病、坏死性牙龈炎、腮腺肿大、卡波西肉瘤、口腔疣和其他疾病。在这里，我们使用454个细菌16S rRNA基因的焦磷酸测序来检查来自HIV阳性和阴性儿童的唾液，粘膜和牙齿样本的口腔微生物组。患者的人口统计学和临床特征收集了一个横截面的患者进行常规牙科护理。对每例患者从口腔不同采样部位采集多份标本。该研究的目的是观察个体患者，样本位置，HIV状态和各种牙齿特征之间口腔微生物群的潜在多样性。我们发现，入组患者之间以及采样地点之间的微生物组存在显著差异。由于患者队列中的不均匀招募，只有5名HIV阴性患者参加了研究，并且在研究构思和完成之间，HIV感染儿童的健康状况迅速改善，使分析变得复杂。艾滋病毒阴性患者的口腔健康状况普遍良好，这限制了可以收集的牙菌斑样本数量。我们没有发现控制良好的HIV阳性患者和HIV阴性对照之间存在显著差异，这表明控制良好的HIV阳性患者与无HIV的患者相比，口腔植物群基本相似。不同牙齿或不同牙齿特征之间的口腔微生物群也没有显着差异。需要进行更多的研究，以更好地描述儿童和艾滋病毒感染控制不良的儿童的口腔微生物组。
The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed population, the spectrum of potential oral disease is even broader, encompassing candidiasis, necrotizing gingivitis, parotid gland enlargement, Kaposi’s sarcoma, oral warts and other diseases. Here, we used 454 pyrosequencing of bacterial 16S rRNA genes to examine the oral microbiome of saliva, mucosal and tooth samples from HIV-positive and negative children. Patient demographics and clinical characteristics were collected from a cross-section of patients undergoing routine dental care. Multiple specimens from different sampling sites in the mouth were collected for each patient. The goal of the study was to observe the potential diversity of the oral microbiota among individual patients, sample locations, HIV status and various dental characteristics. We found that there were significant differences in the microbiome among the enrolled patients, and between sampling locations. The analysis was complicated by uneven enrollment in the patient cohorts, with only five HIV-negative patients enrolled in the study and by the rapid improvement in the health of HIV-infected children between the time the study was conceived and completed. The generally good oral health of the HIV-negative patients limited the number of dental plaque samples that could be collected. We did not identify significant differences between well-controlled HIV-positive patients and HIV-negative controls, suggesting that well-controlled HIV-positive patients essentially harbor similar oral flora compared to patients without HIV. Nor were significant differences in the oral microbiota identified between different teeth or with different dental characteristics. Additional studies are needed to better characterize the oral microbiome in children and those with poorly-controlled HIV infections.
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影响因子：
7.6
作者：
Kumar, PS;Griffen, AL;Leys, EJ
通讯作者：
Leys, EJ
影响因子：
7
作者：
Nasidze, Ivan;Li, Jing;Stoneking, Mark
通讯作者：
Stoneking, Mark
DOI：
10.1016/j.tripleo.2004.10.011
发表时间：
2005-12-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Navazesh, M;Mulligan, R;Slots, J
通讯作者：
Slots, J
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.4
作者：
Kazor, CE;Mitchell, PM;Paster, BJ
通讯作者：
Paster, BJ