A time memory engram embedded in a light-entrainable circadian clock
A time memory engram embedded in a light-entrainable circadian clock
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嵌入光诱导生物钟的时间记忆印迹
DOI：
10.1016/j.cub.2023.10.027
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发表时间：
2023
期刊：
影响因子：
9.2
通讯作者：
Yamazaki, Shin
中科院分区：
文献类型：
--
作者：
Ehichioya, David E.;Taufique, S.K. Tahajjul;Farah, Sofia;Yamazaki, Shin
食物可携带振荡器（FEO）的位置和分子机制是时间生物学中一个长期未解之谜。1,2,3 FEO是一个神秘的昼夜节律起搏器，控制食物预期活动（FAA）。FEO被认为是一个昼夜节律振荡器，它会影响进食时间。然而，FEO的节律特性仍然是一个谜，部分原因是由于区分FAA和由视交叉上核（SCN）主要昼夜节律起搏器控制的运动活动的技术限制。为了克服这一限制，我们使用喂养实验设备版本3 （FED3）来测量寻找食物，戳鼻子的行为。当食物供应限制在夜间4小时时，小鼠在用餐前表现出强烈的预期性戳鼻子行为。当食物供应转移到白天时，小鼠迅速表达了白天预期的鼻子戳，而没有表现出短暂性。出乎意料的是，即使食物颗粒不再在夜间分发，老鼠也会在夜间保持预期的鼻子戳。接下来，我们在不改变用餐时间的情况下，通过改变光-暗循环，测试食物预期是否直接编码在光可携带的振荡器上。预期行为与光暗周期同步变化，尽管进餐时间不变。接下来，我们通过研究1/2/3期三敲除小鼠的SCN功能受损，来测试是否需要一个功能性的SCN来编码预期的戳鼻进餐时间。剔周小鼠的食物预期鼻戳与光-暗周期平行移动，独立于功能性SCN时钟。我们的数据表明，食物预期时间嵌入在一种新颖的、超scn光可携带的振荡器中。
A longstanding mystery in chronobiology is the location and molecular mechanism of the food-entrainable oscillator (FEO).1,2,3The FEO is an enigmatic circadian pacemaker that controls food anticipatory activity (FAA). The FEO is implicated as a circadian oscillator that entrains to feeding time. However, the rhythmic properties of the FEO remain a mystery in part due to technical limitations in distinguishing FAA from locomotor activity controlled by the primary circadian pacemaker in the suprachiasmatic nucleus (SCN). To overcome this limitation, we used the Feeding Experimentation Device version 3 (FED3) to measure food-seeking, nose-poking behavior. When food availability was limited to 4 h at night, mice exhibited strong anticipatory nose-poking behavior prior to mealtime. When food availability was moved to the daytime, mice quickly expressed daytime anticipatory nose pokes without displaying transients. Unexpectedly, the mice also maintained nighttime anticipatory nose pokes, even though food pellets were no longer dispensed at night. We next tested if food anticipation was directly encoded on a light-entrainable oscillator by shifting the light-dark cycle without changing mealtime. Anticipatory behavior shifted in parallel with the light-dark cycle, although meal timing was unchanged. Next, we tested whether encoding meal timing for anticipatory nose pokes required a functional SCN by studyingPeriod 1/2/3triple knockout mice with disabled SCN. Food anticipatory nose poking ofPeriodknockout mice shifted in parallel with the light-dark cycle independent of a functional SCN clock. Our data suggest that food anticipation time is embedded in a novel, extra-SCN light-entrainable oscillator.
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影响因子：
4.6
作者：
Flôres DE;Bettilyon CN;Yamazaki S
通讯作者：
Yamazaki S
影响因子：
4.6
作者：
通讯作者：
--
视交叉上病变大鼠食物获取 T 周期所伴随的活动的强制解离
影响因子：
3.5
作者：
F. Stephan
通讯作者：
F. Stephan
视交叉上病变大鼠的多次喂养活动夹带
影响因子：
2.9
作者：
F. Stephan
通讯作者：
F. Stephan
影响因子：
3.3
作者：
Angeles-Castellanos, M.;Salgado-Delgado, R.;Escobar, C.
通讯作者：
Escobar, C.