Bacteriome and mycobiome associations in oral tongue cancer.
Bacteriome and mycobiome associations in oral tongue cancer.
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DOI：
10.18632/oncotarget.21921
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发表时间：
2017-11-14
期刊：
影响因子：
--
通讯作者：
Eng C
中科院分区：
文献类型：
--
作者：
Mukherjee PK;Wang H;Retuerto M;Zhang H;Burkey B;Ghannoum MA;Eng C
口腔（移动的）舌鳞状细胞癌（OMTC）是一种非人乳头状瘤病毒相关的口腔癌，在没有明确病因的情况下迅速增加。口腔卫生不良与口腔癌有关，这表明口腔细菌组（细菌群落）和真菌组（真菌群落）可能起作用。虽然细菌组越来越多地被认为是健康的积极参与者，但真菌组的作用尚未在OMTC中研究。从39例OMTC患者的成对肿瘤和邻近正常组织中提取组织DNA。微生物组谱、主坐标和相异性指数分析显示，与其匹配的非肿瘤组织相比，肿瘤组织（TT）中的细菌多样性和丰富度以及真菌丰富度显著降低（NTT，P<0.006）。厚壁菌门是最丰富的细菌门，与NTT相比，TT中的厚壁菌门显著增加（分别为48%和40%; P=0.004）。与匹配的NTT相比，TT中拟杆菌和梭杆菌的数量显著减少（两者均P≤0.003）。22种细菌和7种真菌的检出率在TT组和NTT组之间有显著性差异，其中链球菌的检出率最高，在肿瘤组中明显增高（34% vs.22%，P<0.001）。TT中曲霉属真菌的检出率与细菌（放线菌、普雷沃菌、链球菌）呈负相关，与聚集菌属呈正相关。与低T分期患者相比，高T分期患者TT和NTT之间的平均差异较低（0.07 vs. 0.21，P=0.04）。我们的研究结果表明，OMTC与其匹配的正常口腔上皮之间的细菌组和真菌组的差异，以及它们与T分期的相关性。
Squamous cell carcinoma of the oral (mobile) tongue (OMTC), a non-human papilloma virus-associated oral cancer, is rapidly increasing without clear etiology. Poor oral hygiene has been associated with oral cancers, suggesting that oral bacteriome (bacterial community) and mycobiome (fungal community) could play a role. While the bacteriome is increasingly recognized as an active participant in health, the role of the mycobiome has not been studied in OMTC. Tissue DNA was extracted from 39 paired tumor and adjacent normal tissues from patients with OMTC. Microbiome profiling, principal coordinate, and dissimilarity index analyses showed bacterial diversity and richness, and fungal richness, were significantly reduced in tumor tissue (TT) compared to their matched non-tumor tissues (NTT, P<0.006). Firmicutes was the most abundant bacterial phylum, which was significantly increased in TT compared to NTT (48% vs. 40%, respectively; P=0.004). Abundance of Bacteroidetes and Fusobacteria were significantly decreased in TT compared to matched NTT (P≤0.003 for both). Abundance of 22 bacterial and 7 fungal genera was significantly different between the TT and NTT, including Streptococcus, which was the most abundant and significantly increased in the tumor group (34% vs. 22%, P<0.001). Abundance of fungal genus Aspergillus in TT correlated negatively with bacteria (Actinomyces, Prevotella, Streptococcus), but positively with Aggregatibacter. Patients with high T-stage disease had lower mean differences between TT and NTT compared with patients with low T-stage disease (0.07 vs. 0.21, P=0.04). Our results demonstrate differences in bacteriome and mycobiome between OMTC and their matched normal oral epithelium, and their association with T-stage.
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影响因子：
15.9
作者：
Huffnagle, Gary B.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.
影响因子：
6.4
作者：
Ghannoum M
通讯作者：
Ghannoum M
DOI：
10.1007/978-1-4939-3052-4_9
发表时间：
2016-01-01
期刊：
CANDIDA SPECIES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Chandra, Jyotsna;Retuerto, Mauricio;Ghannoum, Mahmoud
通讯作者：
Ghannoum, Mahmoud
影响因子：
7
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
通讯作者：
Holt, Robert A.
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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