Improved recellularization of ex vivo vascular scaffolds using directed transport gradients to modulate ECM remodeling.
Improved recellularization of ex vivo vascular scaffolds using directed transport gradients to modulate ECM remodeling.
复制标题
DOI：
10.1002/bit.24934
复制
发表时间：
2013-07
影响因子：
3.8
通讯作者：
McFetridge, Peter S.
中科院分区：
文献类型：
--
作者：
Tosun, Zehra;McFetridge, Peter S.
从无细胞的体外组织中再生具有功能的、临床上可行的组织一直是个问题，这主要是由于限制细胞迁移和整合的糟糕的营养运输条件。使这些问题复杂化的是亚细胞孔洞大小，为了细胞迁移和组织再生，必须重塑细胞外基质(ECM)。本工作的目的是创造一个定向生长的环境，使细胞能够完全填充体外衍生的血管支架，并在更长的时间内保持活性。设计了三种不同的培养条件，分别使用单(一个营养源)和双灌流生物反应器系统(两个营养源)，以评估压力和营养梯度在低(50/30 mm Hg)或高(120/80)相对压力条件下的影响。使用胶原/水凝胶细胞输送系统将人肌成纤维细胞种植到体外衍生的血管支架的开口部周围。培养30d后，各组间细胞密度基本一致，但由于灌流条件的不同，细胞分布和结构力学存在显著差异。最具侵略性的运输梯度是由单次灌流低压回路形成的，导致细胞通过支架向管腔(营养源)迁移的比例更高。这些研究表明定向营养梯度的影响，其中精确控制的灌流条件显著影响细胞的迁移、分布和功能，从而在早期重建事件中对构建力学产生显著影响。
The regeneration of functional, clinically viable, tissues from acellular ex vivo tissues has been problematic largely due to poor nutrient transport conditions that limit cell migration and integration. Compounding these issues are subcellular pore sizes that necessarily requires extracellular matrix (ECM) remodeling in order for cells to migrate and regenerate the tissue. The aim of the present work was to create a directed growth environment that allows cells to fully populate an ex vivo-derived vascular scaffold and maintain viability over extended periods. Three different culture conditions using single (one nutrient source) or dual perfusion bioreactor systems (two nutrients sources) were designed to assess the effect of pressure and nutrient gradients under either low (50/30 mmHg) or high (120/80) relative pressure conditions. Human myofibroblasts were seeded to the ablumenal periphery of an ex vivo-derived vascular scaffold using a collagen/hydrogel cell delivery system. After 30 days culture, total cell density was consistent between groups; however, significant variation was noted in cell distribution and construct mechanics as a result of differing perfusion conditions. The most aggressive transport gradient was developed by the single perfusion low-pressure circuits and resulted in a higher proportion of cells migrating across the scaffold toward the vessel lumen (nutrient source). These investigations illustrate the influence of directed nutrient gradients where precisely controlled perfusion conditions significantly affects cell migration, distribution and function, resulting in pronounced effects on construct mechanics during early remodeling events.
登录
查看更多内容
影响因子：
4.1
作者：
Abousleiman, Rita I.;Reyes, Yuliana;Sikavitsas, Vassilios
通讯作者：
Sikavitsas, Vassilios
影响因子：
14
作者：
He, Wei;Nieponice, Alejandro;Soletti, Lorenzo;Hong, Yi;Gharaibeh, Burhan;Crisan, Mihaela;Usas, Arvydas;Peault, Bruno;Huard, Johnny;Wagner, William R.;Vorp, David A.
通讯作者：
Vorp, David A.
影响因子：
2.4
作者：
Abousleiman, Rita I.;Reyes, Yuliana;Sikavitsas, Vassilios
通讯作者：
Sikavitsas, Vassilios
影响因子：
4.1
作者：
Heine, Joerg;Schmiedl, Andreas;Kallenbach, Klaus
通讯作者：
Kallenbach, Klaus
影响因子：
4.9
作者：
Moore, Marc;Sarntinoranont, Malisa;McFetridge, Peter
通讯作者：
McFetridge, Peter