Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development.
Characterizations of a Class-I BASIC PENTACYSTEINE Gene Reveal Conserved Roles in the Transcriptional Repression of Genes Involved in Seed Development.
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I 类基本五半胱氨酸基因的表征揭示了种子发育相关基因转录抑制中的保守作用
DOI：
10.3390/cimb44090278
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发表时间：
2022-09-07
影响因子：
3.1
通讯作者：
Yin, Hengfu
中科院分区：
文献类型：
--
作者：
Ma, Xianjin;Yu, Yifan;Hu, Zhikang;Huang, Hu;Li, Sijia;Yin, Hengfu
花器官的发育调控涉及花同源基因的时空调控。基础五半胱氨酸基因是植物特异性转录因子，通过基因转录调控参与植物发育的许多方面。虽然研究表明BPC基因参与花器官的发育调控，但对其在重瓣花形成中的作用知之甚少。本文从观赏花山茶中分离了一类BPC基因（CjBPC1）。结果表明，CjBPC1在单株和双株花的中心轮中均有高表达。CjBPC1在拟南芥中过表达，导致植株和种子严重缺陷。我们发现，涉及胚珠和种子发育的基因，包括SEEDSTICK、LEAFY COTYLEDON2、ABSCISIC ACID INSENSITIVE 3和FUSCA3，在转基因品系中显著下调。我们发现，这些下游基因的组蛋白3赖氨酸27甲基化水平在转基因植株中得到提高，表明CjBPC1在募集Polycomb Repression Complex进行基因抑制中的保守作用。
The developmental regulation of flower organs involves the spatio-temporal regulation of floral homeotic genes. BASIC PENTACYSTEINE genes are plant-specific transcription factors that is involved in many aspects of plant development through gene transcriptional regulation. Although studies have shown that the BPC genes are involved in the developmental regulation of flower organs, little is known about their role in the formation of double-flower due. Here we characterized a Class I BPC gene (CjBPC1) from an ornamental flower—Camellia japonica. We showed that CjBPC1 is highly expressed in the central whorls of flowers in both single and doubled varieties. Overexpression of CjBPC1 in Arabidopsis thaliana caused severe defects in siliques and seeds. We found that genes involved in ovule and seed development, including SEEDSTICK, LEAFY COTYLEDON2, ABSCISIC ACID INSENSITIVE 3 and FUSCA3, were significantly down-regulated in transgenic lines. We showed that the histone 3 lysine 27 methylation levels of these downstream genes were enhanced in the transgenic plants, indicating conserved roles of CjBPC1 in recruiting the Polycomb Repression Complex for gene suppression.
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影响因子：
7.2
作者：
Meister, RJ;Williams, LA;Gasser, CS
通讯作者：
Gasser, CS
影响因子：
6.9
作者：
Simonini S;Kater MM
通讯作者：
Kater MM
DOI：
10.1080/23802359.2021.1987169
发表时间：
2021
期刊：
Mitochondrial DNA. Part B, Resources
影响因子：
--
作者：
Liu Y;Xu Y;Jia X
通讯作者：
Jia X
影响因子：
7.2
作者：
Mozgova, Iva;Koehler, Claudia;Hennig, Lars
通讯作者：
Hennig, Lars
影响因子：
14.9
作者：
Bailey TL;Johnson J;Grant CE;Noble WS
通讯作者：
Noble WS