Genotypic and phenotypic characterization of the California 99 (Cal99) variant of infectious bronchitis virus.
Genotypic and phenotypic characterization of the California 99 (Cal99) variant of infectious bronchitis virus.
复制标题
DOI：
10.1007/s11262-006-0014-7
复制
发表时间：
2007-06
期刊：
影响因子：
1.6
通讯作者：
Cardona CJ
中科院分区：
文献类型：
--
作者：
Mondal SP;Cardona CJ
传染性支气管炎病毒 (IBV) 的加州 99 (Cal99) 变种于 1999 年首次从加州中部接种疫苗的肉鸡群中发现。 Cal99基因组的S1高变区与阿肯色(Ark)血清型病毒关系最为密切。在这项研究中，对Cal99的完整基因组进行了测序，并将结构蛋白基因与常用的IBV疫苗的结构蛋白基因以及世界不同地区自然发生的疫情分离株的结构蛋白基因进行了比较，以阐明遗传物质的潜在来源。根据序列比较，原型Cal99病毒与非致病性ArkDPI病毒相似，不同之处在于S1基因以及S2和M结构蛋白基因的序列片段，分别与康涅狄格州（Conn）和马萨诸塞州（Mass）病毒株更相关。我们推测这两个片段分别来自 Conn 病毒和 Mass 病毒，并被整合到主要源自 ArkDPI 的病毒中。由于Ark、Conn和Mass毒株在加州同时用作活疫苗，疫苗株之间的点突变和重组可能导致了Cal99变异病毒的出现。对 6 个 Cal99 分离株的结构蛋白基因的分析表明，该血清型病毒在非 S1 结构基因方面可能存在显着差异。最后，我们用 Cal99 进行了挑战研究，证明该病毒会导致迟发性呼吸道疾病，其严重程度与 M41 IBV 挑战株相当。
The California 99 (Cal99) variant of infectious bronchitis virus (IBV) was first recovered in 1999 from vaccinated broiler chicken flocks in Central California. The S1 hypervariable region of Cal99 genome was most closely related to Arkansas (Ark) serotype viruses. In this study, the complete genome of Cal99 was sequenced, and the structural protein genes were compared with those of commonly used IBV vaccines as well as those of isolates from naturally occurring outbreaks in different parts of the world, to elucidate potential sources of genetic material. Based on sequence comparison, the prototype Cal99 virus is similar to the apathogenic ArkDPI virus, except in the S1 gene and stretches of sequence in the S2 and M structural protein genes, which are more related to Connecticut (Conn) and Massachusetts (Mass) strain viruses, respectively. We speculate that these two fragments came from a Conn and a Mass virus, respectively, and were incorporated into a virus largely derived from ArkDPI. Since Ark, Conn and Mass strains have been simultaneously used as live vaccines in California, both point mutations and recombination among vaccine strains may have contributed to the emergence of the Cal99 variant virus. Analysis of the structural protein genes of six Cal99 isolates demonstrated that viruses of this serotype may differ substantially in the non-S1 structural genes. Finally, we performed a challenge study with Cal99 and demonstrated that the virus causes late-onset respiratory disease, with a severity comparable to that of the M41 IBV challenge strain.
登录
查看更多内容
影响因子：
1.4
作者：
AVELLANEDA, GE;VILLEGAS, P;KING, DJ
通讯作者：
KING, DJ
影响因子：
5
作者：
Cavanagh D;Davis PJ;Mockett AP
通讯作者：
Mockett AP
影响因子：
1.6
作者：
Callison, SA;Jackwood, MW;Hilt, DA
通讯作者：
Hilt, DA
影响因子：
3.8
作者：
KOCH, G;HARTOG, L;VANROOZELAAR, DJ
通讯作者：
VANROOZELAAR, DJ
影响因子：
1.4
作者：
Sung, HW;Reddy, SM;Fadly, AM
通讯作者：
Fadly, AM