Measures of anxiety in zebrafish (Danio rerio): dissociation of black/white preference and novel tank test.

Measures of anxiety in zebrafish (Danio rerio): dissociation of black/white preference and novel tank test.
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斑马鱼(Danio Rerio)的焦虑度量:黑色/白色偏好和新型坦克测试的解离。

DOI:
10.1371/journal.pone.0036931
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Rosemberg DB
Rosemberg DB
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Blaser RE;Rosemberg DB

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在斑马鱼中评估了墙壁颜色刺激对潜水的影响,以及深度刺激对趋暗性的影响。三组鱼被限制在一个黑色,一个白色,或一个透明的坦克,并测试深度偏好。两组鱼被限制在一个深或浅的坦克,并测试黑白偏好。正如预测的那样,鱼喜欢深的一半,而不是浅的一半,并喜欢黑色/白色坦克的黑色的一半,而不是白色的一半。结果表明,缸壁颜色对深度偏好有显著影响,透明缸产生的深度偏好最强,黑色缸产生的深度偏好最弱。然而,坦克深度并没有显着影响颜色偏好。此外,壁的颜色显着影响穿梭和不动,而深度显着影响穿梭和趋触性。这些结果是一致的,与以前的迹象表明,潜水反应和趋暗性可能反映分离的行为机制。我们的结论是,这两个测试是互补的,而不是互换的,并在两个测试中的行为的动机系统的进一步研究是必要的。
The effects of wall color stimuli on diving, and the effects of depth stimuli on scototaxis, were assessed in zebrafish. Three groups of fish were confined to a black, a white, or a transparent tank, and tested for depth preference. Two groups of fish were confined to a deep or a shallow tank, and tested for black-white preference. As predicted, fish preferred the deep half of a split-tank over the shallow half, and preferred the black half of a black/white tank over the white half. Results indicated that the tank wall color significantly affected depth preference, with the transparent tank producing the strongest depth preference and the black tank producing the weakest preference. Tank depth, however, did not significantly affect color preference. Additionally, wall color significantly affected shuttling and immobility, while depth significantly affected shuttling and thigmotaxis. These results are consistent with previous indications that the diving response and scototaxis may reflect dissociable mechanisms of behavior. We conclude that the two tests are complementary rather than interchangeable, and that further research on the motivational systems underlying behavior in each of the two tests is needed.
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