An automated predator avoidance task in zebrafish.
An automated predator avoidance task in zebrafish.
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DOI：
10.1016/j.bbr.2010.07.028
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
--
作者：
Ahmed, Omar;Seguin, Diane;Gerlai, Robert
斑马鱼在行为神经科学中越来越受欢迎，因为研究人员已经开始意识到专门为该物种开发的复杂遗传工具以及已经可用于其他实验室模型生物的药理学工具的好处。斑马鱼被提议作为分析脊椎动物恐惧反应以及焦虑等人类心理病理状况的体内工具。我们一直在为斑马鱼开发行为任务，可用于筛选突变或药物引起的恐惧反应变化。在本文中，我们提出了先前开发的捕食者回避范式的修改版本，该范式现在允许诱导和量化我们以前无法引发的回避反应。最重要的是，在当前的范式中，斑马鱼现在被证明可以通过增加与图像的距离来响应同域捕食者印度叶鱼的移动图像的出现，这是一种很容易以自动化方式量化的强大反应。然而，出乎意料的是，另一种恐惧反应，即“潜水”反应，仅在测试开始时才出现强烈的反应，而不是对捕食者刺激的反应。我们讨论了这些结果的含义，并得出结论，尽管斑马鱼的恐惧反应很复杂并且依赖于环境，但当前的范式是朝着高通量筛选斑马鱼恐惧反应改变的方向迈出的重要一步。
Zebrafish are becoming increasingly popular in behavioral neuroscience as investigators have started to realize the benefits of sophisticated genetic tools specifically developed for this species along with the pharmacological tools already available for other laboratory model organisms. The zebrafish has been proposed as an in vivo tool for the analysis of vertebrate fear responses as well as human psychopathological conditions such as anxiety. We have been developing behavioral tasks for zebrafish that could be utilized for screening mutation or drug induced changes in fear responses. In this paper we present a modified version of a previously developed predator avoidance paradigm that now allows the induction and quantification of avoidance reactions that we previously could not elicit. Most importantly, in the current paradigm zebrafish are now shown to respond to the appearance of a moving image of a sympatric predator, the Indian leaf fish, by increasing their distance from the image, a robust reaction that is easy to quantify in an automated manner. Unexpectedly, however, another fear response, the “diving” response, was seen robustly only at the beginning of the test but not in response to the predator stimulus. We discuss the implications of these results and conclude that although zebrafish fear responses are complex and context dependent, the current paradigm is a significant step towards high throughput screening for alterations in fear responses of zebrafish.
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影响因子：
2.7
作者：
Egan, Rupert J.;Bergner, Carisa L.;Hart, Peter C.;Cachat, Jonathan M.;Canavello, Peter R.;Elegante, Marco F.;Elkhayat, Salem I.;Bartels, Brett K.;Tien, Anna K.;Tien, David H.;Mohnot, Sopan;Beeson, Esther;Glasgow, Eric;Amri, Hakima;Zukowska, Zofia;Kalueff, Allan V.
通讯作者：
Kalueff, Allan V.
DOI：
10.1016/j.pnpbp.2003.09.012
发表时间：
2003-12-01
影响因子：
5.6
作者：
Blanchard, DC;Griebel, G;Blanchard, RJ
通讯作者：
Blanchard, RJ
影响因子：
8.2
作者：
Rosen, Jeffrey B.;Pagani, Jerome H.;Davis, Cameron
通讯作者：
Davis, Cameron
影响因子：
2.7
作者：
Gerlai, Robert;Fernandes, Yohaan;Pereira, Terence
通讯作者：
Pereira, Terence
影响因子：
2.7
作者：
Bass, Stephanie L. S.;Gerlai, Robert
通讯作者：
Gerlai, Robert