Correlation of corneal immune cell changes with clinical severity in dry eye disease: An in vivo confocal microscopy study.
Correlation of corneal immune cell changes with clinical severity in dry eye disease: An in vivo confocal microscopy study.
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角膜免疫细胞与干眼病中临床严重程度的变化的相关性：体内共聚焦显微镜研究。
DOI：
10.1016/j.jtos.2020.05.012
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Hamrah P
中科院分区：
文献类型：
--
作者：
Aggarwal S;Kheirkhah A;Cavalcanti BM;Cruzat A;Jamali A;Hamrah P
目的：利用活体共聚焦显微镜（IVCM）评价干眼病（DED）患者角膜免疫树突状细胞（DC）的变化，并将IVCM参数与临床严重程度相关联。这是一项回顾性横断面研究，包括150例DED患者的300只眼和49例年龄匹配的对照组的49只眼。DED的严重程度基于干眼研讨会（DEWS）分类。分析中央角膜基底层下的IVCM图像的DC密度和形态（包括每个DC的树突数量、DC大小和DC场）。与对照组相比，DED中的DC密度显著更高（93.4 ± 6.3 vs. 25.9 ± 3.9 cells/mm 2; P < 0.001）。在形态学上，DED组树突数目、DC大小和视野（3.3 ± 0.1，106.9 ± 4.7 μm2，403.8 ± 20.1 μm2）明显大于对照组（2.3 ± 0.1，62.5 ± 5.7 μm2，241.4 ± 24.4 μm2，P < 0.001）。早在1级DED严重程度时就观察到与对照相比显著更高的DC密度（87 ± 10个细胞/mm 2，p < 0.001）。对于树突和DC场，分别在水平2至4（p= < 0.001和p = < 0.05）检测到DC的显著形态学变化。类似地，DC大小在DED水平3-4显示出显著增加。（p < 0.05）。线性回归分析显示结膜和角膜染色与DC密度独立相关，而角膜染色与DC形态独立相关。DC密度和形态与DED的临床严重程度相关。轻度DED患者DC密度增高，重度DED患者DC密度增高。IVCM可能是检测早期免疫变化的有力工具，并可补充DED的临床检查。
To evaluate corneal immune dendritiform cell (DC) changes in dry eye disease (DED) using in vivo confocal microscopy (IVCM) and to correlate IVCM parameters with clinical severity. This was a retrospective, cross-sectional study including 300 eyes of 150 DED patients and 49 eyes of 49 age-matched controls. Severity of DED was based on the Dry Eye Workshop (DEWS) classification. IVCM images of subbasal layer of the central cornea were analyzed for DC density and morphology (including number of dendrites per DC, DC size and DC field). DC density was significantly higher in DED compared to controls (93.4 ± 6.3 vs. 25.9 ± 3.9 cells/ mm2; P < 0.001). Morphologically, number of dendrites, DC size and field were significantly larger in DED (3.3 ± 0.1, 106.9 ± 4.7 μm2, 403.8 ± 20.1 μm2 than controls (2.3 ± 0.1, 62.5 ± 5.7 μm2, 241.4 ± 24.4 μm2, P < 0.001). Significantly higher DC density compared to controls was observed as early as Level 1 DED severity (87 ± 10 cells/mm2, p < 0.001. Significant morphological changes in DC were detected for Levels 2 to 4 (p= < 0.001, and p = < 0.05) for dendrites and DC field, respectively. Similarly, DC size showed significant increase at DED level 3–4. (p < 0.05). Linear regression analysis showed that both conjunctival and corneal staining were independently associated with DC density, while corneal staining was independently associated with DC morphology. DC density and morphology correlated with clinical severity of DED. While, DC density is increased in mild DED, morphological changes are seen only in severe cases. IVCM may be a powerful tool to detect early immune changes and may complement clinical examination in DED.
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影响因子：
4.5
作者：
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通讯作者：
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影响因子：
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影响因子：
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影响因子：
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