Animated bird silhouette above the tank: acute alcohol diminishes fear responses in zebrafish.
Animated bird silhouette above the tank: acute alcohol diminishes fear responses in zebrafish.
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DOI：
10.1016/j.bbr.2012.01.021
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发表时间：
2012-04-01
影响因子：
2.7
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
--
作者：
Luca, Ruxandra M.;Gerlai, Robert
酒精依赖和酒精滥用是未得到满足的主要医疗需求。斑马鱼被认为是研究酒精对脑功能和行为的影响及其机制的一种有前途的脊椎动物。众所周知，酒精会引起运动功能的改变以及恐惧和焦虑。在这里，我们提出了一个最近开发的恐惧范例，我们采用了一个动画（移动）图像的鸟的轮廓。我们测量了急性酒精给药（剂量范围：0.00 - 0.75体积/体积百分比，浴暴露60分钟）对斑马鱼行为反应的影响。我们测试这些反应在一个前刺激，刺激和后刺激期间的任务，使用视频跟踪和基于观察的量化方法。发现恐惧诱导刺激减少了斑马鱼与水箱底部的距离，增加了不稳定运动的数量，并增加了酒精暴露鱼（与对照鱼相比）的跳跃数量。酒精以剂量依赖的方式减弱这些恐惧反应。此外，酒精在最高剂量下降低了一般活动，这种影响与刺激的呈现无关。我们讨论了观察和视频跟踪的结果之间的相似性和差异，并得出结论，恐惧范式将是有用的，揭示酒精诱导的功能变化，在斑马鱼的大脑。
Alcohol dependence and alcohol abuse represent major unmet medical needs. The zebrafish is considered to be a promising vertebrate species with which the effects of alcohol on brain function and behavior and the mechanisms underlying these effects may be studied. Alcohol is known to induce alterations in motor function as well as fear and anxiety. Here we present a recently developed fear paradigm in which we employ an animated (moving) image of a bird silhouette. We measure the effect of acute alcohol administration (dose range employed: 0.00 – 0.75 vol/vol percentage, bath exposure for 60 minutes) on the behavioral responses of zebrafish. We test these responses during a pre-stimulus, stimulus and post-stimulus period of the task using both a video-tracking and an observation based quantification method. The fear inducing stimulus was found to decrease the distance of the zebrafish from the bottom of the tank, to increase number of erratic movements, and to increase the number of jumps in alcohol exposed fish (versus control fish). Alcohol attenuated these fear responses in a dose dependent manner. In addition, alcohol decreased general activity at the highest dose, an effect that was independent of the presentation of the stimulus. We discuss the similarities and differences between observation and video-tracking based results and conclude that fear paradigms will be useful in revealing alcohol induced functional changes in the brain of zebrafish.
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影响因子：
2.7
作者：
Egan, Rupert J.;Bergner, Carisa L.;Hart, Peter C.;Cachat, Jonathan M.;Canavello, Peter R.;Elegante, Marco F.;Elkhayat, Salem I.;Bartels, Brett K.;Tien, Anna K.;Tien, David H.;Mohnot, Sopan;Beeson, Esther;Glasgow, Eric;Amri, Hakima;Zukowska, Zofia;Kalueff, Allan V.
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Kalueff, Allan V.
影响因子：
5.3
作者：
Gilman, Jodi M.;Ramchandani, Vijay A.;Hommer, Daniel W.
通讯作者：
Hommer, Daniel W.
影响因子：
6.1
作者：
Da Silva, GE;Vendruscolo, LF;Takahashi, RN
通讯作者：
Takahashi, RN
影响因子：
2.9
作者：
Blaser, R. E.;Penalosa, Y. M.
通讯作者：
Penalosa, Y. M.
影响因子：
3.6
作者：
Blaser, Rachel E.;Koid, Audrey;Poliner, Rebecca M.
通讯作者：
Poliner, Rebecca M.