Utility of Light-Adapted Full-Field Electroretinogram ON and OFF Responses for Detecting Glaucomatous Functional Damage.
Utility of Light-Adapted Full-Field Electroretinogram ON and OFF Responses for Detecting Glaucomatous Functional Damage.
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DOI：
10.1167/tvst.12.8.16
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发表时间：
2023-08-01
影响因子：
3
通讯作者：
Fortune, Brad
中科院分区：
文献类型：
--
作者：
Dunn, Michaela;Cull, Grant;Reynaud, Juan;Jennings, Dawn;Holthausen, Trinity;Di Polo, Adriana;Fortune, Brad
关键词：
比较视网膜电图（ERG）反应参数检测非人灵长类动物（NHP）实验性青光眼（EG）早期功能丧失的能力，包括对蓝色背景上标准短暂红色闪光（R/B）和200 ms长R/B和白色白色（W/W）闪光的明视觉负反应（PhNR），以及对W/W闪烁刺激（5-50 Hz）的反应，以及暗适应强度系列。在12只麻醉猴中记录了单侧EG的光适应ERG。测量了a波、b波和d波的振幅和隐式时间，以及PhNR的振幅和闪光起始和偏移的振荡电位。采用峰谷和基频分析测量闪烁ERG。暗适应ERG参数由Naka-Rushton关系建模。只有PhNR振幅显着降低EG的眼睛相比，其他控制（FC）的眼睛。仅W/W长闪光时EG眼与FC眼的d波隐式时间延迟，但在所有眼中，R/B比W/W条件下慢10 ~ 20 ms。与FC相比，EG的闪烁ERG总体延迟<0.5 ms，但振幅仅在5 Hz时受到影响。简短的R/B PhNR振幅检测EG的灵敏度最高，最强的相关性参数的结构损伤。PhNR的标准简短R/B刺激是最好的检测和以下的早期功能丧失NHP EG。这些结果表明，在青光眼的临床管理中，使用较长持续时间的闪光来分离发作和抵消反应没有益处。
To compare parameters of electroretinogram (ERG) responses for their ability to detect functional loss in early stages of nonhuman primate (NHP) experimental glaucoma (EG), including photopic negative responses (PhNR) to a standard brief red flash on a blue background (R/B) and 200-ms-long R/B and white-on-white (W/W) flashes, to W/W flicker stimuli (5–50 Hz), and to a dark-adapted intensity series. Light-adapted ERGs were recorded in 12 anesthetized monkeys with unilateral EG. Amplitudes and implicit times of the a-wave, b-wave, and d-wave were measured, as well as amplitudes of PhNRs and oscillatory potentials for flash onset and offset. Flicker ERGs were measured using peak–trough and fundamental frequency analyses. Dark-adapted ERG parameters were modeled by Naka–Rushton relationships. Only PhNR amplitudes were significantly reduced in EG eyes compared to fellow control (FC) eyes. The d-wave implicit time was delayed in EG versus FC eyes only for the W/W long flash, but in all eyes it was 10 to 20 ms slower for R/B versus the W/W condition. Flicker ERGs were <0.5 ms delayed in EG versus FC overall, but amplitudes were affected only at 5 Hz. The brief R/B PhNR amplitude had the highest sensitivity to detect EG and strongest correlation to parameters of structural damage. The PhNR to the standard brief R/B stimulus was best for detecting and following early-stage functional loss in NHP EG. These results suggest that there would be no benefit in using longer duration flashes to separate onset and offset responses for clinical management of glaucoma.
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影响因子：
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