3D imaging of transition metals in the zebrafish embryo by X-ray fluorescence microtomography.
3D imaging of transition metals in the zebrafish embryo by X-ray fluorescence microtomography.
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DOI：
10.1039/c4mt00121d
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Fahrni CJ
中科院分区：
文献类型：
--
作者：
Bourassa D;Gleber SC;Vogt S;Yi H;Will F;Richter H;Shin CH;Fahrni CJ
同步x射线荧光（SXRF）微层析成像已成为生物样品中元素分布三维可视化的一种强大技术。在获得层析投影系列时，仪器和标本的机械稳定性是至关重要的。通过将渐进降温法（PLT）与飞秒激光切片相结合，我们能够在受精后24小时将斑马鱼胚胎嵌入、切除并保存在x射线兼容的透明树脂中，用于层析元素成像。基于在100小时光束时间内以2 μm步长获得的60个投影数据集，我们使用迭代最大似然期望最大化（MLEM）重建算法重建了锌、铁和铜的三维分布。每一种过渡金属的体积元素图包含了超过1.24亿个体素，揭示了不同的元素分布，这些元素分布可能与胚胎发育阶段的特征解剖特征相关。
Synchrotron X-ray fluorescence (SXRF) microtomography has emerged as a powerful technique for the 3D visualization of the elemental distribution in biological samples. The mechanical stability, both of the instrument and the specimen, is paramount when acquiring tomographic projection series. By combining the progressive lowering of temperature method (PLT) with femtosecond laser sectioning, we were able to embed, excise, and preserve a zebrafish embryo at 24 hours post fertilization in an X-ray compatible, transparent resin for tomographic elemental imaging. Based on a data set comprised of 60 projections, acquired with a step size of 2 μm during 100 hours of beam time, we reconstructed the 3D distribution of zinc, iron, and copper using the iterative maximum likelihood expectation maximization (MLEM) reconstruction algorithm. The volumetric elemental maps, which entail over 124 million individual voxels for each transition metal, revealed distinct elemental distributions that could be correlated with characteristic anatomical features at this stage of embryonic development.
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作者：
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DOI：
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