Integration of contractile forces during tissue invagination.
Integration of contractile forces during tissue invagination.
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DOI：
10.1083/jcb.200910099
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发表时间：
2010-03-08
期刊：
影响因子：
--
通讯作者：
Wieschaus EF
中科院分区：
文献类型：
--
作者：
Martin AC;Gelbart M;Fernandez-Gonzalez R;Kaschube M;Wieschaus EF
转录因子 Twist 促进细胞连接，将单个细胞连接成一个收缩网络，负责上皮形态发生过程中的顶端收缩脉冲。在上皮形态发生过程中，单个细胞内的肌动球蛋白细胞骨架产生的收缩力共同产生组织水平的力。在果蝇中胚层内陷期间，脉冲肌动球蛋白网状收缩和细胞形状的棘轮状稳定驱动顶端收缩。在这里，我们研究收缩力如何在组织中整合。降低粘附连接（AJ）水平或消融肌动球蛋白网会导致组织范围的上皮撕裂，从而释放主要沿前后（a-p）胚胎轴定向的张力。上皮撕裂使通常沿着 a-p 轴伸长的细胞能够各向同性收缩，这表明顶端收缩产生各向异性上皮张力，从而反馈控制细胞形状。上皮张力需要转录因子 Twist，它可以稳定顶端肌球蛋白 II，促进细胞上肌动球蛋白网络的形成，其中径向肌动球蛋白纤维在点 AJ 处首尾相连。因此，脉冲式肌动球蛋白收缩需要细胞上的拉伸网状结构，以在形态发生过程中将细胞力传递到组织水平。
Transcription factor Twist promotes cell junctions to link individual cells into a contractile network responsible for the apical constriction pulses during epithelial morphogenesis. Contractile forces generated by the actomyosin cytoskeleton within individual cells collectively generate tissue-level force during epithelial morphogenesis. During Drosophila mesoderm invagination, pulsed actomyosin meshwork contractions and a ratchet-like stabilization of cell shape drive apical constriction. Here, we investigate how contractile forces are integrated across the tissue. Reducing adherens junction (AJ) levels or ablating actomyosin meshworks causes tissue-wide epithelial tears, which release tension that is predominantly oriented along the anterior–posterior (a-p) embryonic axis. Epithelial tears allow cells normally elongated along the a-p axis to constrict isotropically, which suggests that apical constriction generates anisotropic epithelial tension that feeds back to control cell shape. Epithelial tension requires the transcription factor Twist, which stabilizes apical myosin II, promoting the formation of a supracellular actomyosin meshwork in which radial actomyosin fibers are joined end-to-end at spot AJs. Thus, pulsed actomyosin contractions require a supracellular, tensile meshwork to transmit cellular forces to the tissue level during morphogenesis.
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影响因子：
64.8
作者：
Martin, Adam C.;Kaschube, Matthias;Wieschaus, Eric F.
通讯作者：
Wieschaus, Eric F.
影响因子：
9.2
作者：
Landsberg, Katharina P.;Farhadifar, Reza;Dahmann, Christian
通讯作者：
Dahmann, Christian
影响因子：
14.9
作者：
Arziman Z;Horn T;Boutros M
通讯作者：
Boutros M
影响因子：
64.5
作者：
COSTA, M;WILSON, ET;WIESCHAUS, E
通讯作者：
WIESCHAUS, E
DOI：
10.1083/jcb.149.2.471
发表时间：
2000-04-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
通讯作者：
Montague RA