Maternal separation stress in male mice: long-term increases in alcohol intake.
Maternal separation stress in male mice: long-term increases in alcohol intake.
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DOI：
10.1007/s00213-008-1307-4
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发表时间：
2008-12
影响因子：
3.4
通讯作者：
Miczek, Klaus A.
中科院分区：
文献类型：
--
作者：
Cruz, Fabio C.;Quadros, Isabel M.;Planeta, Cleopatra da S.;Miczek, Klaus A.
关键词：
在啮齿类动物中，长期的母体分离被用作影响成年药物摄入的发育早期环境应激模型。本研究的目的是评估长期母亲分离对酒精消耗的长期影响，使用两种不同的自我管理程序的小鼠：操作性酒精自我管理与三瓶选择。从出生后第1天（PND）至第14天，每天将幼仔与母鼠分离（母体分离，MS）180分钟或不受干扰，仅在笼清洁期间进行处理（动物设施饲养，AFR）。在PND 60，他们被分配到两个实验操作之一：三瓶选择或操作性口服酒精自我管理。在三瓶选择程序中，给予小鼠6%或10%的酒精或0.05%的糖精溶液，每天2小时，持续10天。在第二个实验中，小鼠通过口服酒精（6%或10%的糖精）或0.05%的糖精溶液在每天30分钟的会议期间加强鼻子戳。在获取阶段之后，确定了“断点”。随后，允许小鼠接触强化溶液1小时，没有剂量限制。在三瓶选择程序中，MS小鼠在10%酒精浓度下的酒精摄入量高于AFR。在操作性酒精自我管理，MS小鼠实现更高的酒精摄入量比AFR在浓度为6%和10%，在1小时的会议。结果表明，MS对雄性小鼠酒精摄入量的长期影响，表明早期生活压力是酒精消费和滥用的风险因素。
In rodents, prolonged maternal separation has been used as a model of developmentally early environmental stress to influence adult drug intake. The aim of the present study was to evaluate the long-term effects of prolonged maternal separation on alcohol consumption using two different self-administration procedures in mice: operant alcohol self-administration vs. three-bottle choice. From postnatal day (PND) 1 to 14, pups were separated from the dam (maternal separation, MS) daily for 180 min or were left undisturbed, only handled during cage cleaning (animal facility rearing, AFR). On PND 60, they were assigned to one of two experimental manipulations: either a three-bottle choice or operant oral alcohol self-administration. In the three-bottle choice procedure, mice were given access to 6% or 10% alcohol or 0.05% saccharin solution for 2 h/day for 10 days. In the second experiment, mice were reinforced for nose poking by delivery of oral alcohol (6% or 10% in saccharin) or 0.05% saccharin solutions during daily 30-min sessions. Following the acquisition phase, “break points” were determined. Later, mice were allowed 1 h access to the reinforcing solution with no dosage limitation. In the three-bottle choice procedure, MS mice showed higher alcohol intake than AFR at the 10% alcohol concentration. In the operant alcohol self-administration, MS mice achieved higher alcohol intake than AFR at the concentrations 6% and 10% during the 1-h session. The results demonstrate the long-term consequences of MS on alcohol intake in male mice, suggesting early life stress as a risk factor for alcohol consumption and abuse.
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DOI：
10.1111/j.1530-0277.2006.00247.x
发表时间：
2006-12-01
影响因子：
3.2
作者：
Gustafsson, Lisa;Nylander, Ingrid
通讯作者：
Nylander, Ingrid
影响因子：
2.7
作者：
Boyce-Rustay, Janel M.;Janos, Alicia L.;Holmes, Andrew
通讯作者：
Holmes, Andrew
影响因子：
2.3
作者：
Bowers, WJ;Sabongui, AG;Amit, Z
通讯作者：
Amit, Z
影响因子：
3.7
作者：
Levine, S
通讯作者：
Levine, S
DOI：
10.15288/jsa.1985.46.72
发表时间：
1985-01-01
期刊：
JOURNAL OF STUDIES ON ALCOHOL
影响因子：
--
作者：
LINSKY, AS;STRAUS, MA;COLBY, JP
通讯作者：
COLBY, JP