A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse.
A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse.
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VPm 中继突触突触特性快速改变的关键时期
DOI：
10.3389/fnmol.2017.00238
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发表时间：
2017
影响因子：
4.8
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Pan L;Yang J;Yang Q;Wang X;Zhu L;Liu Y;Lou H;Xu C;Shen Y;Wang H
除了皮质区域，丘脑在生命早期的关键时期也表现出可塑性。由于大多数感觉信息通过丘脑传递到皮层，因此了解丘脑中这一关键时期的精确时间窗和潜在机制将具有重要意义。通过在急性脑切片中使用体外全细胞膜片记录，我们发现VPm中继突触仅对出生后第11天（P11）至P14的胡须剥夺敏感。在P11至P14窗口内开始的胡须剥夺显著降低了AMPAR-EPSC的振幅，但在胡须去除后24小时记录时，NMDAR-EPSC的振幅没有降低。从P10到P11，进入关键期的时间和NMDAR-EPSCs功能的动力学发生了显著改变。在P11，NMDAR-EPSC对含NR 2B的NMDAR的选择性阻断剂ifenprodil的敏感性降低，与P10相比，NR 2A的蛋白水平显著升高。在关键期结束时，P14和P15之间的突触特性没有明显的变化。使用钙成像，我们发现，更少的P15 VPm神经元可以兴奋的GABA α受体激动剂，蝇蕈醇，当与P14 VPm神经元相比，这与KCC 2表达的增加。我们的研究揭示了一个精确的关键时期的感觉经验依赖的可塑性在丘脑具有不同的分子机制，发生在这个关键窗口的开始和结束。
In addition to cortical areas, the thalamus also displays plasticity during a critical period in early life. Since most sensory information is transmitted to the cortex via the thalamus, it will be of significant interest to understand the precise time window and underlying mechanisms of this critical period in the thalamus. By using in vitro whole-cell patch recording in acute brain slices, we found that VPm relay synapses were only sensitive to whisker deprivation from postnatal day 11 (P11) to P14. Whisker deprivation initiated within the P11 to P14 window significantly reduced the amplitude of AMPAR-EPSCs, but not NMDAR-EPSCs when recorded 24 h after whisker removal. From P10 to P11, the timing for entry into the critical period and the kinetics underlying NMDAR-EPSCs function were significantly altered. At P11, NMDAR-EPSCs were less sensitive to ifenprodil, a selective blocker of NR2B-containing NMDAR, and the protein level of NR2A was significantly increased compared to those at P10. At the end of the critical period there were no obvious changes in synaptic properties when compared between P14 and P15. Using calcium imaging, we found that fewer P15 VPm neurons could be excited by the GABAa receptor agonist, muscimol, when compared to P14 VPm neurons; this correlated to an increase in KCC2 expression. Our studies revealed a precise critical period of sensory experience-dependent plasticity in the thalamus featuring distinct molecular mechanisms which occur at the start and end of this critical window.
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